Department of Bacterial Genetics, Faculty of Biology, Institute of Microbiology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland.
Extremophiles. 2012 May;16(3):363-76. doi: 10.1007/s00792-012-0435-2. Epub 2012 Mar 4.
The complete nucleotide sequence of plasmid pP62BP1 (34,467 bp), isolated from Arctic Psychrobacter sp. DAB_AL62B, was determined and annotated. The conserved plasmid backbone is composed of several genetic modules, including a replication system (REP) with similarities to the REP region of the iteron-containing plasmid pPS10 of Pseudomonas syringae. The additional genetic load of pP62BP1 includes two highly related type II restriction-modification systems and a set of genes (slfRCHSL) encoding enzymes engaged in the metabolism of organic sulfates, plus a putative transcriptional regulator (SlfR) of the AraC family. The pP62BP1 slf locus has a compact and unique structure. It is predicted that the enzymes SlfC, SlfH, SlfS and SlfL carry out a chain of reactions leading to the transformation of alkyl sulfates into acyl-CoA, with dodecyl sulfate (SDS) as a possible starting substrate. Comparative analysis of the nucleotide sequences of pP62BP1 and other Psychrobacter spp. plasmids revealed their structural diversity. However, the presence of a few highly conserved DNA segments in pP62BP1, plasmid 1 of P. cryohalolentis K5 and pRWF-101 of Psychrobacter sp. PRwf-1 is indicative of recombinational shuffling of genetic information, and is evidence of lateral gene transfer in the Arctic environment.
已从北极 Psychrobacter sp. DAB_AL62B 中分离得到质粒 pP62BP1（34467bp），并对其进行了全序列测定和注释。该质粒的保守型骨架由多个遗传模块组成，包括与假单胞菌 pPS10 中的含回文序列质粒 REP 区具有相似性的复制系统（REP）。pP62BP1 的附加遗传负荷包括两个高度相关的 II 型限制修饰系统和一组编码参与有机硫酸盐代谢的酶的基因（slfRCHSL），以及假定的 AraC 家族转录调节因子（SlfR）。pP62BP1 的 slf 基因座具有紧凑且独特的结构。据预测，SlfC、SlfH、SlfS 和 SlfL 酶将进行一系列反应，将烷基硫酸盐转化为酰基辅酶 A，十二烷基硫酸盐（SDS）可能是起始底物。对 pP62BP1 与其他 Psychrobacter spp. 质粒的核苷酸序列进行比较分析，揭示了它们的结构多样性。然而，pP62BP1、嗜冷杆菌 K5 质粒 1 和 Psychrobacter sp. PRwf-1 的 pRWF-101 中存在几个高度保守的 DNA 片段，表明遗传信息的重组重排，这是在北极环境中发生水平基因转移的证据。
