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用两种不同固定剂保存人类耳蜗。

Preservation of the human cochlea with two different fixatives.

作者信息

Engström B, Hillerdal M, Hillerdal G

机构信息

Department of Otorhinolaryngology, University Hospital, Uppsala, Sweden.

出版信息

Acta Otolaryngol Suppl. 1990;470:31-3. doi: 10.3109/00016488909138353.

DOI:10.3109/00016488909138353
PMID:2239230
Abstract

The cochlear morphology of eight inner ears from 4 patients was studied by electron microscopy. The perilymphatic perfusion was performed in 2 of them 2 and 2 1/2 h and in 2 others, 10 and 11 h post mortem. In each patient, Karnovsky's fixative was used in one ear and Britta's fixative in the other. No structural differences between the two ears were ascertained in the patients with a short post-mortem fixation time. The cochleas with 2 h post-mortem fixation time were better preserved than those with the 10 and 11 h delay. The cochleas with the longer delay from death to perilymphatic perfusion were still fairly well preserved. However, in these cochleas the stronger fixative (Britta's fixative) gave a better preservation.

摘要

通过电子显微镜研究了4例患者8个内耳的耳蜗形态。其中2例在死后2小时和2个半小时进行了外淋巴灌注,另外2例在死后10小时和11小时进行了外淋巴灌注。在每例患者中,一只耳朵使用卡诺夫斯基固定液,另一只耳朵使用布里塔固定液。在死后固定时间较短的患者中,未确定两只耳朵之间存在结构差异。死后固定2小时的耳蜗比延迟10小时和11小时的耳蜗保存得更好。从死亡到外淋巴灌注延迟时间较长的耳蜗仍保存得相当好。然而,在这些耳蜗中,更强效的固定液(布里塔固定液)保存效果更好。

相似文献

1
Preservation of the human cochlea with two different fixatives.用两种不同固定剂保存人类耳蜗。
Acta Otolaryngol Suppl. 1990;470:31-3. doi: 10.3109/00016488909138353.
2
Preservation of the human cochlea.人类耳蜗的保存。
Ann Otol Rhinol Laryngol. 1985 May-Jun;94(3):284-92.
3
Scanning electron microscopy of the human cochlea--postmortem autolysis artefacts.人类耳蜗的扫描电子显微镜检查——死后自溶假象
Arch Otorhinolaryngol. 1980;228(1):1-6. doi: 10.1007/BF00455888.
4
The influence of different fixatives and osmolality on the ultrastructure of the cochlear neuroepithelium.不同固定剂和渗透压对耳蜗神经上皮超微结构的影响。
Arch Otorhinolaryngol. 1977 Dec 20;218(1-2):67-78. doi: 10.1007/BF00469735.
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The block surface method for evaluation of human inner ears.用于评估人类内耳的块表面法。
Acta Otolaryngol Suppl. 1987;436:25-36. doi: 10.3109/00016488709124973.
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Ultrastruct Pathol. 1997 Mar-Apr;21(2):195-200. doi: 10.3109/01913129709021318.
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