Anniko M, Lundquist P G
Arch Otorhinolaryngol. 1977 Dec 20;218(1-2):67-78. doi: 10.1007/BF00469735.
To investigate which fixative is most suitable for the preservation of cochlear hair cells three main groups of fixatives were used: 1. 3% glutar aldehyde in various buffers (osmolality altered). 2. Glutar aldehyde/paraform aldehyde combination (ad modum Karnovsky). 3. Osmic acid solutions (OsO4) in veronal acetate buffer according to Rhodin (1954). The 2% osmic acid fixative (350--370mosm/kg) resulted in an excellent ultrastructural preservation; an acceptable result was obtained with a 3% glutar aldehyde fixative in 0.1 M sodium phosphate buffer, 600--700 mosm/kg and the Karnovsky's fixative.
为了研究哪种固定剂最适合用于保存耳蜗毛细胞，使用了三大类固定剂：1. 各种缓冲液中的3%戊二醛（渗透压改变）。2. 戊二醛/多聚甲醛组合（改良卡诺夫斯基法）。3. 根据罗丁（1954年）的方法，在醋酸巴比妥缓冲液中使用的锇酸溶液（OsO4）。2%的锇酸固定剂（350 - 370毫渗摩尔/千克）能实现出色的超微结构保存效果；在0.1M磷酸钠缓冲液（600 - 700毫渗摩尔/千克）中的3%戊二醛固定剂以及卡诺夫斯基固定剂也能得到可接受的结果。
