Tamada Yutaka, Takeuchi Hideyuki, Suzuki Nao, Aoki Daisuke, Irimura Tatsuro
Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Tumour Biol. 2012 Aug;33(4):1215-22. doi: 10.1007/s13277-012-0369-4. Epub 2012 Mar 6.
Eight of 15 human ovarian carcinoma cell lines were shown to express high levels of hyaluronan (HA) on their surfaces. The role of cell surface HA in its adhesion to mesothelial cells, which is potentially involved in peritoneal dissemination, was evaluated. Three human ovarian carcinoma cell lines, ES-2, MH, and KF cells, were repeatedly sorted into variant cell lines with high levels of cell surface HA (ES-2/HA+7, MH/HA+7, and KF/HA+7) and with low cell surface HA (ES-2/HA-7, MH/HA-7, and KF/HA-7). The ability of these cells to adhere to peritoneal mesothelial cells was compared. ES-2/HA+7, MH/HA+7, and KF/HA+7 cells were less adherent to mesothelial cells than the ES-2/HA-7, MH/HA-7, and KF/HA-7 cells. On ovarian carcinoma cells, high cell surface HA levels seem to inversely correlate with their capacity to adhere and disseminate to the peritoneum. Considering that peritoneum implantation is the primary ovarian cancer complication, HA cell surface expression may be considered a property associated with a less aggressive phenotype, which is contrary to the general perception that HA expression is associated with malignant progression.
15种人卵巢癌细胞系中有8种被证明在其表面高水平表达透明质酸（HA）。评估了细胞表面HA在其与间皮细胞黏附中的作用，间皮细胞黏附可能与腹膜播散有关。将三种人卵巢癌细胞系ES-2、MH和KF细胞反复分选成细胞表面HA水平高的变异细胞系（ES-2/HA+7、MH/HA+7和KF/HA+7）和细胞表面HA水平低的变异细胞系（ES-2/HA-7、MH/HA-7和KF/HA-7）。比较了这些细胞与腹膜间皮细胞黏附的能力。ES-2/HA+7、MH/HA+7和KF/HA+7细胞比ES-2/HA-7、MH/HA-7和KF/HA-7细胞对间皮细胞的黏附性更低。在卵巢癌细胞上，细胞表面高水平的HA似乎与其黏附及播散至腹膜的能力呈负相关。考虑到腹膜种植是卵巢癌的主要并发症，HA的细胞表面表达可能被认为是一种与侵袭性较低表型相关的特性，这与HA表达与恶性进展相关的普遍观念相反。
