Kokenyesi R
Department of Obstetrics, Gynecology and Women's Health, Saint Louis University, St. Louis, Missouri 63117, USA.
J Cell Biochem. 2001;83(2):259-70. doi: 10.1002/jcb.1230.
Metastatic ovarian carcinoma metastasizes by intra-peritoneal, non-hematogenous dissemination. The adhesion of the ovarian carcinoma cells to extracellular matrix components, such as types I and III collagen and cellular fibronectin, is essential for intra-peritoneal dissemination. The purpose of this study was to determine whether cell surface proteoglycans (a class of matrix receptors) are produced by ovarian carcinoma cells, and whether these proteoglycans have a role in the adhesion of ovarian carcinoma cells to types I and III collagen and fibronectin. Proteoglycans were metabolically labeled for biochemical studies. Both phosphatidylinositol-anchored and integral membrane-type cell surface proteoglycans were found to be present on the SK-OV-3 and NIH:OVCAR-3 cell lines. Three proteoglycan populations of differing hydrodynamic size were detected in both SK-OV-3 and NIH:OVCAR-3 cells. Digestions with heparitinase and chondroitinase ABC showed that cell surface proteoglycans of SK-OV-3 cells had higher proportion of chondroitin sulfate proteoglycans (75:25 of chondroitin sulfate:heparan sulfate ratio), while NIH:OVCAR-3 cells had higher proportion of heparan sulfate proteoglycans (10:90 of chondroitin sulfate:heparan sulfate ratio). RT-PCR indicated the synthesis of a unique assortment of syndecans, glypicans, and CD44 by the two cell lines. In adhesion assays performed on matrix-coated titer plates both cell lines adhered to types I and III collagen and cellular fibronectin, and cell adhesion was inhibited by preincubation of the matrix with heparin, heparan sulfate, chondroitin sulfate, dermatan sulfate, or chondroitin glycosaminoglycans. Treatment of the cells with heparitinase, chondroitinase ABC, or methylumbelliferyl xyloside also interfered with adhesion confirming the role of both heparan sulfate and chondroitin sulfate cell surface proteoglycans as matrix receptors on ovarian carcinoma cells.
转移性卵巢癌通过腹膜内而非血行播散进行转移。卵巢癌细胞与细胞外基质成分（如I型和III型胶原蛋白以及细胞纤连蛋白）的黏附对于腹膜内播散至关重要。本研究的目的是确定卵巢癌细胞是否产生细胞表面蛋白聚糖（一类基质受体），以及这些蛋白聚糖在卵巢癌细胞与I型和III型胶原蛋白及纤连蛋白的黏附中是否发挥作用。对蛋白聚糖进行代谢标记以用于生化研究。在SK-OV-3和NIH:OVCAR-3细胞系中均发现了磷脂酰肌醇锚定型和整合膜型细胞表面蛋白聚糖。在SK-OV-3和NIH:OVCAR-3细胞中均检测到三种具有不同流体力学大小的蛋白聚糖群体。用硫酸乙酰肝素酶和软骨素酶ABC消化显示，SK-OV-3细胞的细胞表面蛋白聚糖中硫酸软骨素蛋白聚糖比例较高（硫酸软骨素与硫酸乙酰肝素的比例为75:25），而NIH:OVCAR-3细胞中硫酸乙酰肝素蛋白聚糖比例较高（硫酸软骨素与硫酸乙酰肝素的比例为10:90）。逆转录聚合酶链反应表明这两种细胞系合成了独特的一组多配体蛋白聚糖、磷脂酰肌醇蛋白聚糖和CD44。在基质包被的滴度板上进行的黏附试验中，两种细胞系均黏附于I型和III型胶原蛋白以及细胞纤连蛋白，并且通过用肝素、硫酸乙酰肝素、硫酸软骨素、硫酸皮肤素或硫酸软骨素糖胺聚糖对基质进行预孵育可抑制细胞黏附。用硫酸乙酰肝素酶、软骨素酶ABC或甲基伞形酮基木糖苷处理细胞也会干扰黏附，证实了硫酸乙酰肝素和硫酸软骨素细胞表面蛋白聚糖作为卵巢癌细胞上基质受体的作用。
