Department of Environmental Engineering, Zhejiang University, No.866 Yuhangtang Road, Hangzhou 310058, People's Republic of China.
Environ Sci Pollut Res Int. 2012 Sep;19(8):3185-91. doi: 10.1007/s11356-012-0822-3. Epub 2012 Mar 6.
An aerobic denitrifier was isolated from the Hua-Jia-Chi pond in China and identified as Pseudomonas mendocina 3-7 (Genbank No. HQ285879). This isolated strain could express periplasmic nitrate reductase which is essential for aerobic denitrification occurred when the dissolved oxygen (DO) level maintains at 3-10 mg L(-1).
To determine whether the ability of isolated strain is exhibited in the bioremediation of polluted drinking source water, the heterotrophic nitrification and aerobic denitrification characteristics of P. mendocina 3-7 under different cultural conditions such as oxygen level, nitrate and organic concentrations were studied from the nitrogenous balance in the paper.
By measuring the nitrogen balance in all experiments under different culture conditions, the removal of total organic carbon and ammonium was positively correlated with total nitrogen removal, especially under high substrate level. With substrate concentration decreasing, ammonium and nitrate removal occurred separately, and ammonium was completely utilized first under low substrate concentration. Compared to that under high substrate level, the specific growth rate of P. mendocina 3-7 was not low under the low substrate level and the pollutant removal efficiencies remained high, which implies the stronger nitrogen removal and acclimatization capacities of the strain in oligotrophic niches.
从中国华侨池分离出一株好氧反硝化菌，鉴定为假单胞菌 Mendocina 3-7（Genbank No. HQ285879）。该分离株可表达周质硝酸盐还原酶，这对于溶解氧（DO）水平维持在 3-10mg/L 时发生的好氧反硝化是必需的。
为了确定分离株是否具有在污染饮用水源的生物修复中的能力，从氮平衡的角度研究了 P. mendocina 3-7 在不同培养条件下（如氧水平、硝酸盐和有机浓度）的异养硝化和好氧反硝化特性。
通过测量不同培养条件下所有实验中的氮平衡，总有机碳和铵的去除与总氮去除呈正相关，特别是在高基质水平下。随着基质浓度的降低，铵和硝酸盐的去除分别发生，在低基质浓度下首先完全利用铵。与高基质水平相比，P. mendocina 3-7 在低基质水平下的比生长速率并不低，污染物去除效率仍然很高，这意味着该菌株在贫营养生境中具有更强的氮去除和适应能力。
