Wen Gang, Wang Tong, Huang Ting-Lin, Wan Qi-Qi, Cao Rui-Hua, Kou Li-Qing, Yang Shang-Ye
Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Huan Jing Ke Xue. 2020 May 8;41(5):2339-2348. doi: 10.13227/j.hjkx.201911201.
Due to the problems of low nitrogen removal efficiency and unclear electron transfer during biological denitrification treatment of an oligotrophic drinking water reservoir, the nitrogen removal characteristics, environmental adaptability, and electron transfer during denitrification were systematically studied using the aerobic denitrifier ZMF5, which has efficient nitrogen removal ability. The results showed that:① Strain ZMF5 exhibited efficient heterotrophic nitrification and aerobic denitrification ability, with an ammonia removal rate of 0.211 mg·(L·h) and a nitrate removal rate of 0.236 mg·(L·h), and the nitrification intermediates were not accumulated during the treatment process. ② According to analysis of the nitrogen removal efficiency and growth kinetics of strain ZMF5, the strain can effectively utilize different types of carbon source, and show efficient nitrogen removal efficiency under the conditions of low C/N, low pH, and low temperature. ③ Analysis of nitrogen balance showed that carboxylate compound, compared with carbohydrate, could promote the process of aerobic denitrification and change the nitrogen removal pathway of strain ZMF5, i.e., 38.81% of nitrogen was transformed into gas, higher than the 29.81% for assimilation. ④ Analysis of carbon balance indicated that most carbon sources were used as electron donors in the denitrification process, but fewer electrons were used for nitrate reduction, and with respect to different carbon sources, electron transfer to the nitrate respiratory chain was regulated by different reduction potentials, electron donor abundance, and molecular weight. ZMF5 could be used to control nitrogen pollution in drinking water reservoirs.
针对贫营养型饮用水水库生物反硝化处理中存在的脱氮效率低和电子传递不明确的问题，利用具有高效脱氮能力的好氧反硝化菌ZMF5，系统研究了反硝化过程中的脱氮特性、环境适应性及电子传递。结果表明：①菌株ZMF5表现出高效的异养硝化和好氧反硝化能力，氨去除率为0.211 mg·(L·h)，硝酸盐去除率为0.236 mg·(L·h)，处理过程中硝化中间产物未积累。②通过对菌株ZMF5的脱氮效率和生长动力学分析可知，该菌株能有效利用不同类型的碳源，在低C/N、低pH和低温条件下表现出高效的脱氮效率。③氮平衡分析表明，与碳水化合物相比，羧酸盐化合物能促进好氧反硝化过程，并改变菌株ZMF5的脱氮途径，即38.81%的氮转化为气体，高于同化作用的29.81%。④碳平衡分析表明，大多数碳源在反硝化过程中用作电子供体，但用于硝酸盐还原的电子较少，对于不同的碳源，电子向硝酸盐呼吸链的传递受不同还原电位、电子供体丰度和分子量的调节。ZMF5可用于控制饮用水水库中的氮污染。
