Henry Wellcome Laboratories for Integrated Neuroscience and Endocrinology, University of Bristol, Dorothy Hodgkin Building, Whitson Street, Bristol BS1 3NY, United Kingdom.
J Biol Chem. 2012 Apr 27;287(18):14726-33. doi: 10.1074/jbc.M111.326041. Epub 2012 Mar 5.
We show that a single gene locus gives rise to two fully processed and functional miRNAs, i.e. that due to imperfect base pairing, two distinct microRNAs (miRNAs) can be produced from the fully complementary DNA strands. The antisense strand encodes miR-214, which is transcribed by its own promoter, whereas a novel miRNA, miR-3120, is co-expressed with its host gene mRNA. We also found that miR-3120 regulates important aspects of cellular function that are similar to that of its host gene, dynamin-3. miR-3120 was found to be located in neuronal cell bodies and to target Hsc70 and auxilin, and its lentivirus-mediated expression inhibited the uncoating of clathrin-coated vesicles. Finally, mirror miRNAs are likely to represent a new group of miRNAs with complex roles in coordinating gene expression.
我们证明了一个基因座可以产生两个完全加工和功能的 miRNA，即由于不完全碱基配对，可以从完全互补的 DNA 链产生两个不同的 microRNA（miRNA）。反义链编码 miR-214，它由自己的启动子转录，而一种新的 miRNA，miR-3120，与宿主基因 mRNA 共同表达。我们还发现 miR-3120 调节细胞功能的重要方面与其宿主基因 dynamin-3 相似。发现 miR-3120 位于神经元细胞体中，靶向 Hsc70 和 auxilin，其慢病毒介导的表达抑制了网格蛋白包被小泡的脱壳。最后，mirror miRNAs 可能代表一组具有复杂调节基因表达作用的新 miRNA。
