Wang Yiwei, Zhang Yingfeng, Li Yujie, Yang Weipeng, Wang Yanli, Chen Ying, Yang Qing, Li Tao, Dong Yu, Zhu Xiaoxin
China Academy of Chinese Medical Sciences, Institute of Chinese Materia Medica, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2011 Dec;36(23):3319-26.
To research the representative ingredient excretion in bile using the different compatibilities of Wuji Wan, in order to indicate the regularity of compatibility.
L9 (3(4)) orthogonal design table was used, in addition 9 simples, altogether 18 compatibilities. After duodenal administration bile at different time spot was collected for LC-MS detection.
The excretion of Evodiae Fructus was negative correlated with that of berberine and palmatine in bile. The excretion of Paeoniae Radix Alba was positive correlated with that of berberine and palmatine in bile. The excretion of Coptidis Rhizoma, Paeoniae Radix Alba was negative correlated with that of evodiamine, rutaecarpine; meanwhile, the most superior proportion was also caculated which promote the representative ingredient excrete from bile.
Evodiae fructus suppresses representative ingredients of Coptidis Rhizoma excrete through bile. Paeoniae Radix Alba promote suppresses representative ingredients of Coptidis Rhizoma excrete through bile. Coptidis Rhizoma, paeoniae Radix Alba suppresses representative ingredients of Evodiae Fructus excrete through bile. Coptidis Rhizoma, Evodiae Fructus suppresses representative ingredients of Paeoniae Radix Alba excrete through bile.
研究戊己丸不同配伍组合中代表性成分的胆汁排泄情况，以揭示配伍规律。
采用L9(3(4))正交设计表，9味单味药，共18种配伍组合。十二指肠给药后，在不同时间点收集胆汁进行LC-MS检测。
吴茱萸在胆汁中的排泄与小檗碱和巴马汀呈负相关。白芍在胆汁中的排泄与小檗碱和巴马汀呈正相关。黄连、白芍在胆汁中的排泄与吴茱萸碱、吴茱萸次碱呈负相关；同时，计算出促进代表性成分从胆汁中排泄的最优比例。
吴茱萸抑制黄连代表性成分经胆汁排泄。白芍促进黄连代表性成分经胆汁排泄。黄连、白芍抑制吴茱萸代表性成分经胆汁排泄。黄连、吴茱萸抑制白芍代表性成分经胆汁排泄。
