Pan Hua-zheng, Liang Jun, Yu Zhuang, Lun Li-min, Li Hui, Wang Qing
Department of Laboratory, Medical College of Qingdao University, Qingdao, China.
Asian Pac J Cancer Prev. 2011;12(11):2947-50.
We conducted a case-control study in China to clarify the association between the XRCC1-Arg399Gln polymorphism and HCC risk.
A total of 202 cases and 236 controls were selected from the the Affiliated Hospital of Qingdao University from May 2008 to May 2010. Assessment of the XRCC1-Arg399Gln polymorphism was based upon duplex polymerase-chain-reactions with the confronting-two-pair primer (PCR- CTPP) method. All analyses were performed using the STATA statistical package.
A significant increase in risk was associated with the Arg/Gln genotype (adjusted OR 1.55, 95%CI=1.03-2.57) compared with Arg/ Arg. However, the Gln/Gln genotype had non-significant increased risk of HCC with adjusted OR (95%CI) of 1.34(0.67-2.38). There was also a significant increase with the Arg/Gln genotype among HCC patients above 50 years old (OR=1.95, 95% CI=1.14-3.57). Additionally, the risk of HCC was moderately increased in drinkers with Arg/Gln genotype compared with never drinkers, and the adjusted OR (95% CI) was 1.89 (1.13-3.45).
This study demonstrated that a polymorphism in a DNA repair gene may influence the risk of HCC. The XRCC1 codon Arg/Gln was this associated with an increased risk of HCC, especially in patients above 50 years old and/or with a drinking habit.
我们在中国开展了一项病例对照研究，以阐明XRCC1基因Arg399Gln多态性与肝癌风险之间的关联。
2008年5月至2010年5月期间，从青岛大学附属医院选取了202例病例和236例对照。采用两对引物对抗PCR法（PCR-CTPP）对XRCC1基因Arg399Gln多态性进行评估。所有分析均使用STATA统计软件包进行。
与Arg/Arg基因型相比，Arg/Gln基因型的风险显著增加（校正比值比为1.55，95%置信区间为1.03-2.57）。然而，Gln/Gln基因型的肝癌风险虽有增加但无统计学意义，校正比值比（95%置信区间）为1.34（0.67-2.38）。在50岁以上的肝癌患者中，Arg/Gln基因型的风险也显著增加（比值比=1.95，95%置信区间为1.14-3.57）。此外，与从不饮酒者相比，携带Arg/Gln基因型的饮酒者患肝癌的风险中度增加，校正比值比（95%置信区间）为1.89（1.13-3.45）。
本研究表明，DNA修复基因中的多态性可能影响肝癌风险。XRCC1基因密码子Arg/Gln与肝癌风险增加相关，尤其是在50岁以上和/或有饮酒习惯的患者中。
