Samanian Sara, Mahjoubi Frouzandeh, Mahjoubi Bahar, Mirzaee Rezvan, Azizi Rasool
Department of Clinical Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Asian Pac J Cancer Prev. 2011;12(11):3141-5.
Over-expression of some genes, such as MDR1, can increase the level of chemotherapy drug afflux and limit the effectiveness of treatment. We aimed to investigate the effect of MDR1 polymorphisms on its expression level and related characteristics in Iranian colorectal cancer patients.
Tumor, normal mucosal tissue and blood samples from CRC patients and blood samples from healthy controls (n=60) were obtained for genotyping and measuring the expression level of MDR1.
The expression of the MDR1 gene showed a significant increase in cancerous regions compared to adjacent normal tissue. Furthermore, the GG2677 genotype was correlated with highest while the AT 2677 genotype was associated with the lowest levels of expression. In addition only the G2677T/A polymorphism showed association with histological grade of colorectal tumors.
Our study once more emphasizes effects of MDR1 SNPs which may indirectly impact on response to drugs.
某些基因如MDR1的过度表达可增加化疗药物外排水平并限制治疗效果。我们旨在研究MDR1基因多态性对伊朗结直肠癌患者其表达水平及相关特征的影响。
获取结直肠癌患者的肿瘤、正常黏膜组织和血液样本以及健康对照者的血液样本（n = 60），用于基因分型和测量MDR1的表达水平。
与相邻正常组织相比，MDR1基因在癌组织区域的表达显著增加。此外，GG2677基因型与最高表达水平相关，而AT 2677基因型与最低表达水平相关。另外，只有G2677T/A多态性与结直肠肿瘤的组织学分级相关。
我们的研究再次强调了MDR1单核苷酸多态性的影响，其可能间接影响药物反应。
