基因多态性rs2032582对伊拉克急性髓系白血病患者治疗反应的药代动力学影响
Pharmacokinetic Effect of Gene Polymorphism rs2032582 on the Therapeutic Response in Iraqi Patients with Acute Myeloid Leukemia.
作者信息
Abdulkareem Rafid A, Rafaa Tamadher Abbas, Jasim Hamsa Ahmed, Suleiman Ahmed Abdul Jabbar
机构信息
Institute of Genetic Engineering and Biotechnology for Post Graduate Studies, University of Baghdad, Baghdad, Iraq.
University of Anbar, Anbar, Iraq.
出版信息
Avicenna J Med Biotechnol. 2020 Oct-Dec;12(4):241-245.
BACKGROUND
The main problem in treatment of leukemia patients is the chemotherapy resistance which is a main concern in recent years. The cause of chemotherapy drug resistance is related to gene which is located on chromosome 7 (7q21-31) and it is mainly connected with energy-dependent efflux (P-glycoprotein). This study was conducted to assess the correlation between MDR polymorphism and chemotherapy efficiency with Vincristine in a sample of Iraqi Acute Myeloid Leukemia (AML) patients.
METHODS
The blood sample of 200 AML patients and 200 controls were collected and the frequency of rs2032582 was calculated through sequencing and then the role of different genetic patterns was evaluated on cancer cells by MTT assay.
RESULTS
The results indicate that GG and TT genotypes (20 and 20.5% from total patients count) are more frequent in Iraqi AML patients than other genetic patterns in gene and also the genotype TA is more sensitive to Vincristine chemotherapy than other genotypes.
CONCLUSION
It seems that genetic pattern is the main factor in determination of chemotherapy of AML patients, and patients should not undergo chemotherapy with such drugs, especially Vincristine.
背景
白血病患者治疗中的主要问题是化疗耐药性，这是近年来的主要关注点。化疗药物耐药性的原因与位于7号染色体（7q21 - 31）上的基因有关，且主要与能量依赖性外排（P - 糖蛋白）相关。本研究旨在评估伊拉克急性髓系白血病（AML）患者样本中多药耐药（MDR）基因多态性与长春新碱化疗效果之间的相关性。
方法
收集200例AML患者和200例对照的血样，通过测序计算rs2032582的频率，然后通过MTT法评估不同基因模式对癌细胞的作用。
结果
结果表明，伊拉克AML患者中GG和TT基因型（占患者总数的20%和20.5%）比该基因的其他基因模式更常见，并且TA基因型对长春新碱化疗比其他基因型更敏感。
结论
似乎基因模式是决定AML患者化疗的主要因素，并且患者不应使用此类药物进行化疗，尤其是长春新碱。
Zotero 插件