Aoki K, Noto K, Hasegawa I
Aoki Eye Clinic.
Nippon Ganka Gakkai Zasshi. 1990 Aug;94(8):736-40.
We evaluated enzyme-linked immunosorbent assay (Adenoclone) in 253 cases of epidemic keratoconjunctivitis (EKC), pharyngeal conjunctival fever (PCF) and acute conjunctivitis. The clinical diagnosis due to adenoviral ocular infection was 86/114 (75%) in EKC, 26/50 (52%) in PCF and 25/89 (28%) in acute conjunctivitis, Adenoclone was positive only within 7 days after the disease onset. The specificity (87%) and sensitivity (74%) of Adenoclone were confirmed by isolate cell culture. Cases with corneal complication due to adenoviral infection show higher a value than the OD value in Adenoclone and the bulbar conjunctiva is a suitable site to detect adenoviral antigen. In this survey, isolated adnovirus type 4 was recognized in 53%, Ad8 in 14%, Ad3 in 13%, Ad37 in 5%, Ad19 in 2% and non typed Ad in 12%. Clinical findings due to Ad4 showed EKC in 50%, PCF in 30% and acute conjunctivitis in 20% respectively.
我们对253例流行性角结膜炎（EKC）、咽结膜热（PCF）和急性结膜炎患者进行了酶联免疫吸附测定（Adenoclone）。腺病毒眼部感染的临床诊断在EKC中为86/114（75%），在PCF中为26/50（52%），在急性结膜炎中为25/89（28%）。Adenoclone仅在发病后7天内呈阳性。通过分离细胞培养证实了Adenoclone的特异性（87%）和敏感性（74%）。腺病毒感染导致角膜并发症的病例在Adenoclone中的a值高于OD值，球结膜是检测腺病毒抗原的合适部位。在本次调查中，分离出的腺病毒4型占53%，腺病毒8型占14%，腺病毒3型占13%，腺病毒37型占5%，腺病毒19型占2%，未分型腺病毒占12%。腺病毒4型导致的临床表现分别为50%的EKC、30%的PCF和20%的急性结膜炎。
