Nakagawa H, Ohno S, Isobe Y, Aoki K, Tanifuji Y, Nakagawa Y, Usui M, Usui N, Ando N, Murakata A
Department of Ophthalmology, Tokyo Women's Medical College, Japan.
Nippon Ganka Gakkai Zasshi. 1995 Oct;99(10):1164-9.
We evaluated an enzyme-linked immunosorbent assay test (Adenoclone) for the rapid diagnosis of adenovirus infection in 589 cases of acute follicular conjunctivitis. Of 255 cases of adenoviral conjunctivitis proven by positive virus isolation. Adenoclone was positive in 51.0% by visual determination and 40.4% by spectrophotometry. Twenty-seven of 130 cases giving visually positive results were interpreted to be negative by spectrophotometry. In 334 adenovirus-negative cases, Adenoclone was negative in 99.1% and 99.4 by visual and spectrophotometric determination, respectively. Adenoclone was less sensitive in cases of adenovirus 3, 4 or 37, infections than in those of adenovirus 8, and also showed lower sensitivity in cases presenting mild conjunctivitis. Adenoclone is a rapid and easy test with high specificity but low sensitivity, and thus the test seems to be helpful in diagnosing adenoviral conjunctivitis.
我们评估了一种酶联免疫吸附试验（Adenoclone）在589例急性滤泡性结膜炎患者中快速诊断腺病毒感染的效果。在255例经病毒分离阳性证实为腺病毒性结膜炎的病例中，通过肉眼判定Adenoclone阳性率为51.0%，通过分光光度法测定阳性率为40.4%。在130例肉眼判定为阳性的病例中，有27例经分光光度法测定被判定为阴性。在334例腺病毒阴性的病例中，通过肉眼判定Adenoclone阴性率为99.1%，通过分光光度法测定阴性率为99.4%。Adenoclone对腺病毒3型、4型或37型感染的检测敏感性低于腺病毒8型感染，对轻度结膜炎病例的检测敏感性也较低。Adenoclone是一种快速简便的检测方法，特异性高但敏感性低，因此该检测方法似乎有助于诊断腺病毒性结膜炎。
