Cojocaru Elena, Trandafirescu Mioara, Leon Magda, Cotuţiu C, Foia Liliana
Department of Histology, Grigore T. Popa University of Medicine and Pharmacy, Iassy, Romania.
Rom J Morphol Embryol. 2012;53(1):61-6.
Inflammation of the vascular wall is an essential event in the development of atherosclerosis, the main leukocytes of the inflammatory infiltrate being the monocyte/macrophages. These cells are very heterogeneous and rapidly change their function in response to signals received from the local environment.
The aim of this research was to study the immunohistochemical expression of the anti-CD68 antibody at the level of the atherosclerotic plaque.
We used tissue samples obtained by endarterectomies in patients with symptomatic atherosclerotic lesions. Inflammatory reaction was highlighted in the arterial wall by immunohistochemistry using an anti-CD68 monoclonal antibody that marks normal or pathological monocytes/macrophages.
The types of analyzed atherosclerotic lesions showed a positive reaction with the anti-CD68 antibody, varying in distribution and intensity in the vascular intima. The CD68 was positive in macrophages depending on the extent of the inflammatory reaction.
The presence of many macrophages in the atherosclerotic plaques indicates a chronic inflammatory reaction, accompanied by fibroblast proliferation and connective tissue changes that influence the stability of the plaques.
血管壁炎症是动脉粥样硬化发展过程中的一个重要事件，炎症浸润的主要白细胞是单核细胞/巨噬细胞。这些细胞非常异质，并会根据从局部环境接收到的信号迅速改变其功能。
本研究的目的是研究抗CD68抗体在动脉粥样硬化斑块水平的免疫组化表达。
我们使用了有症状动脉粥样硬化病变患者经动脉内膜切除术获得的组织样本。通过使用标记正常或病理性单核细胞/巨噬细胞的抗CD68单克隆抗体进行免疫组化，突出显示动脉壁中的炎症反应。
所分析的动脉粥样硬化病变类型与抗CD68抗体呈阳性反应，在血管内膜中的分布和强度各不相同。根据炎症反应的程度，巨噬细胞中的CD68呈阳性。
动脉粥样硬化斑块中存在许多巨噬细胞表明存在慢性炎症反应，伴有成纤维细胞增殖和结缔组织变化，这些会影响斑块的稳定性。
