Mezei T, Horváth Emoke, Turcu M, Gurzu Simona, Raica M, Jung I
Department of Pathology, University of Medicine and Pharmacy of Targu Mures, Romania.
Rom J Morphol Embryol. 2012;53(1):67-71.
The growth of solid tumors requires the development of microvessels, therefore tumor expansion depends on angiogenesis. Microvessels provide nutrients and oxygen and remove catabolytic substances, while endothelial cells produce growth factors for tumor cells in a paracrine fashion. The microvascular component of a tumor also plays a role in the metastatic capacity of the tumor, enabling the tumor cells to spread to distant locations by providing a large endothelial surface.
The purpose of this study was to review the literature about angiogenesis regarding malignant lymphomas and to perform basic measurements by means of digital morphometric methods in large B-cell lymphomas and follicular lymphomas.
After thorough analyzing currently available assessment methods, we performed angiogenesis assessment on 19 randomly selected cases, from paraffin-embedded specimens using digital morphometry. We used immunohistochemistry and the CD34 antigen to mark microvessels. We measured average vascular diameter and a previously successfully applied digital morphometric method to quantify the extent of endothelial area.
According to literature data, our knowledge and understanding of angiogenesis grew rapidly from early studies such as Folkman's classic paper. Many studies showed that angiogenesis plays a key role in the biology of tumors and therefore the study of angiogenesis might open new therapeutic possibilities. There have been many studies of angiogenesis in malignant lymphomas, however not as many articles as in other tumor types. Our morphometric studies showed there are statistically significant differences between diffuse large cell lymphoma (DLBCL) and follicular lymphoma (FL) regarding average vascular diameter and that high grade lymphomas tend to have a greater CD34+ endothelial area.
实体瘤的生长需要微血管的形成，因此肿瘤的扩展依赖于血管生成。微血管提供营养物质和氧气，并清除分解代谢产物，而内皮细胞以旁分泌方式产生肿瘤细胞生长因子。肿瘤的微血管成分在肿瘤的转移能力中也起作用，通过提供大的内皮表面使肿瘤细胞扩散到远处。
本研究的目的是回顾有关恶性淋巴瘤血管生成的文献，并通过数字形态计量学方法对大B细胞淋巴瘤和滤泡性淋巴瘤进行基础测量。
在全面分析现有评估方法后，我们从石蜡包埋标本中随机选取19例病例，采用数字形态计量学进行血管生成评估。我们使用免疫组织化学和CD34抗原标记微血管。我们测量了平均血管直径，并采用一种先前成功应用的数字形态计量学方法来量化内皮面积。
根据文献数据，从早期研究如福克曼的经典论文开始，我们对血管生成的认识和理解迅速增长。许多研究表明，血管生成在肿瘤生物学中起关键作用，因此对血管生成的研究可能会开辟新的治疗可能性。已经有许多关于恶性淋巴瘤血管生成的研究，然而文章数量不如其他肿瘤类型多。我们的形态计量学研究表明，弥漫性大细胞淋巴瘤（DLBCL）和滤泡性淋巴瘤（FL）在平均血管直径方面存在统计学显著差异，并且高级别淋巴瘤往往具有更大的CD34+内皮面积。
