人胱抑素 C 在翼状胬肉与健康结膜中的过度表达。

Over-expression of human cystatin C in pterygium versus healthy conjunctiva.

机构信息

Optometry Department, Center for Health Sciences, Autonomous University of Aguascalientes, Aguascalientes, Mexico.

出版信息

BMC Ophthalmol. 2013 Feb 27;13:6. doi: 10.1186/1471-2415-13-6.

DOI:10.1186/1471-2415-13-6

PMID:23442876

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3598526/

Abstract

BACKGROUND

A prospective, non-randomised, transversal and comparative study, carried out in INOVA Vision Institute and Autonomous University of Aguascalientes. Pterygium is an important illness that affects 22% people from tropic and equatorial zones. Is an inflammatory process caused by UV rays, and it has a behavior similar to a neoplasm. For this study was taken into consideration 191 samples from the INOVA Vision Institute, Aguascalientes, Mexico. Include 73 pterygia samples, which were obtained during resection under sterile conditions. 44 normal conjunctiva samples were obtained from the same patients when harvesting the conjunctival autograft, or from other patients undergoing extracapsular cataract extraction from the superior bulbar region. Tears from patients with pterygium (n = 50) and normal volunteers (n = 24) were obtained using a calibrated glass micro capillary tube. The surgical conjunctiva and pterygia samples were subjected to reverse-transcription polymerase chain reaction (RT-PCR), western blot, and immunohistochemistry. Tears were analyzed by enzyme-linked immunosorbent assays.

METHODS

This was a prospective, non-randomised study involving 191 biological samples taken from patients with pterygium and normal volunteers, whom were operated under local anaesthesia by either complete resection of the lesion with primary closure, or resection with conjunctival autograft. Tissue samples were fixed in 10% formaldehyde. Sections were routinely stained with hematoxylin and eosin. HCC expression was evaluated by reverse-transcription polymerase chain reaction (RT-PCR), immunohistochemistry, and by western blotting. All tears samples were analyzed by enzyme-linked immunosorbent assays (ELISA).

RESULTS

Expression levels and distribution patterns of HCC in normal conjunctiva and pterygium. Higher levels of HCC mRNAs and proteins were detected in pterygium compared with a normal conjunctiva. Immunohistochemistry revealed that HCC was localized in the apical cells of the epithelium in the normal conjunctiva. In contrast, HCC was detected in all extension of epithelial tissue, from apical to basal cells in pterygia. The concentration of HCC protein in tears was higher in patients with pterygium versus controls.

CONCLUSION

HCC may play an important role in protecting normal conjunctiva, and regulating inflammatory conditions of the anterior ocular surface.

摘要

背景

这是一项在 INOVA 视觉研究所和阿瓜斯卡连特斯自治大学进行的前瞻性、非随机、横断和对照研究。翼状胬肉是一种重要的疾病，影响 22%的热带和赤道地区人群。它是一种由紫外线引起的炎症过程，其行为类似于肿瘤。在这项研究中，考虑了来自墨西哥阿瓜斯卡连特斯 INOVA 视觉研究所的 191 个样本。其中包括 73 个翼状胬肉样本，这些样本是在无菌条件下切除时获得的。从同一患者中采集结膜自体移植物时，或从其他接受上巩膜区白内障囊外摘除术的患者中，获得了 44 个正常结膜样本。使用校准的玻璃微毛细管管从翼状胬肉患者（n=50）和正常志愿者（n=24）中获得泪液。对手术切除的结膜和翼状胬肉样本进行逆转录聚合酶链反应（RT-PCR）、western blot 和免疫组织化学分析。通过酶联免疫吸附试验（ELISA）分析泪液。

方法

这是一项前瞻性、非随机研究，涉及 191 名翼状胬肉患者和正常志愿者的生物样本，这些患者在局部麻醉下接受手术治疗，包括病变的完全切除和原发性闭合，或切除联合结膜自体移植物。组织样本用 10%甲醛固定。常规用苏木精和伊红染色。通过逆转录聚合酶链反应（RT-PCR）、免疫组织化学和 western blot 评估 HCC 表达。通过酶联免疫吸附试验（ELISA）分析所有泪液样本。

结果

正常结膜和翼状胬肉中 HCC 的表达水平和分布模式。与正常结膜相比，翼状胬肉中 HCC 的 mRNAs 和蛋白质水平更高。免疫组织化学显示 HCC 定位于正常结膜的上皮细胞的顶端细胞。相反，在翼状胬肉中，HCC 检测到上皮组织的所有延伸，从顶端细胞到基底细胞。与对照组相比，翼状胬肉患者的 HCC 蛋白在泪液中的浓度更高。