Department of Mechanical Engineering, University of Washington, Box 352600, Seattle, Washington 98195, USA.
Lab Chip. 2012 Apr 21;12(8):1437-40. doi: 10.1039/c2lc21131a. Epub 2012 Mar 6.
A rapid, accurate tuberculosis diagnostic tool that is compatible with the needs of tuberculosis-endemic settings is a long-sought goal. An immunofluorescence microtip sensor is described that detects Mycobacterium tuberculosis complex cells in sputum in 25 minutes. Concentration mechanisms based on flow circulation and electric field are combined at different scales to concentrate target bacteria in 1 mL samples onto the surfaces of microscale tips. Specificity is conferred by genus-specific antibodies on the microtip surface. Immunofluorescence is then used to detect the captured cells on the microtip. The detection limit in sputum is 200 CFU mL(-1) with a success rate of 96%, which is comparable to PCR.
一种快速、准确且适用于结核病流行地区的结核诊断工具是长期以来的目标。本文描述了一种免疫荧光微尖传感器，它可以在 25 分钟内检测痰液中的结核分枝杆菌复合体细胞。基于流循环和电场的浓缩机制在不同尺度上结合，将 1 毫升样本中的目标细菌浓缩到微尺度尖端的表面。微尖端表面上的种特异性抗体赋予了特异性。然后使用免疫荧光法检测微尖端上捕获的细胞。在痰液中的检测限为 200 CFU mL(-1)，成功率为 96%，与 PCR 相当。
