Department of Bioresource Engineering, Macdonald Campus, McGill University, Montreal, QC, Canada.
J Ind Microbiol Biotechnol. 2012 Jun;39(6):949-55. doi: 10.1007/s10295-012-1094-0. Epub 2012 Mar 7.
Economically viable production of solvents through acetone-butanol-ethanol (ABE) fermentation requires a detailed understanding of Clostridium acetobutylicum. This study focuses on the proteomic profiling of C. acetobutylicum ATCC 824 from the stationary phase of ABE fermentation using xylose and compares with the exponential growth by shotgun proteomics approach. Comparative proteomic analysis revealed 22.9% of the C. acetobutylicum genome and 18.6% was found to be common in both exponential and stationary phases. The proteomic profile of C. acetobutylicum changed during the ABE fermentation such that 17 proteins were significantly differentially expressed between the two phases. Specifically, the expression of five proteins namely, CAC2873, CAP0164, CAP0165, CAC3298, and CAC1742 involved in the solvent production pathway were found to be significantly lower in the stationary phase compared to the exponential growth. Similarly, the expression of fucose isomerase (CAC2610), xylulose kinase (CAC2612), and a putative uncharacterized protein (CAC2611) involved in the xylose utilization pathway were also significantly lower in the stationary phase. These findings provide an insight into the metabolic behavior of C. acetobutylicum between different phases of ABE fermentation using xylose.
通过丙酮丁醇乙醇（ABE）发酵实现溶剂的经济生产需要对丙酮丁醇梭菌（Clostridium acetobutylicum）有详细的了解。本研究使用木糖聚焦于 ABE 发酵稳定期的丙酮丁醇梭菌 ATCC 824 的蛋白质组学分析，并通过 shotgun 蛋白质组学方法与指数生长期进行比较。比较蛋白质组学分析显示，22.9%的丙酮丁醇梭菌基因组和 18.6%在指数生长期和稳定期都有发现。ABE 发酵过程中丙酮丁醇梭菌的蛋白质组图谱发生变化，两个阶段之间有 17 个蛋白质的表达差异显著。具体来说，在稳定期与指数生长期相比，涉及溶剂生产途径的 5 种蛋白质（CAC2873、CAP0164、CAP0165、CAC3298 和 CAC1742）的表达明显降低。同样，木糖利用途径中的果糖异构酶（CAC2610）、木酮糖激酶（CAC2612）和一个假定的未鉴定蛋白（CAC2611）的表达在稳定期也明显降低。这些发现深入了解了使用木糖时 ABE 发酵不同阶段丙酮丁醇梭菌的代谢行为。
