Huang Yan, Zhao Guoqing, Peng Zuogang
The Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Beibei, Chongqing, PR China.
Mitochondrial DNA. 2012 Jun;23(3):173-5. doi: 10.3109/19401736.2012.660935. Epub 2012 Mar 7.
The complete mitogenome sequence of a vulnerable species Onychostoma lini was determined using polymerase chain reaction and directly sequenced with primer walking method. The complete mitogenome was 16,595 bp in length, containing 37 genes of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a control region (D-loop). The gene order and composition of O. lini was similar to most other fishes. The descending order of the base composition on heavy strand was 31.6% A, 27.9% C, 24.6% T, and 15.9% G, with a relatively lower level of G and a slight AT bias of 56.2%. The codon usage followed the typical vertebrate mitochondrial pattern (ATG or GTG for start codon and TAA or TAG for stop codon). The complete mitogenome sequence of O. lini provides fundamental data for further conversation genetics and captive breeding studies on this species.
利用聚合酶链反应测定了易危物种漓江光唇鱼的完整线粒体基因组序列,并采用引物步移法直接测序。完整的线粒体基因组长度为16595bp,包含37个基因,其中有13个蛋白质编码基因、22个tRNA基因、2个rRNA基因和一个控制区(D-loop)。漓江光唇鱼的基因顺序和组成与大多数其他鱼类相似。重链上碱基组成由高到低依次为31.6%A、27.9%C、24.6%T和15.9%G,G含量相对较低,AT偏倚率为56.2%。密码子使用遵循典型的脊椎动物线粒体模式(起始密码子为ATG或GTG,终止密码子为TAA或TAG)。漓江光唇鱼完整的线粒体基因组序列为该物种进一步的保护遗传学和圈养繁殖研究提供了基础数据。