Ma Xiuhui, Huang Fujiang, Wang Zhijian
The Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education) and.
Mitochondrial DNA. 2014 Aug;25(4):245-6. doi: 10.3109/19401736.2013.796517. Epub 2013 Jun 24.
The complete mitogenome sequence of Acrossocheilus monticolus, which is endemic to China, was determined using long PCR reactions. The genome is 16,599 bp in length, including 13 typical vertebrate protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and a control region. Except for eight tRNA and ND6 genes, all other mitochondrial genes are encoded on the heavy strand. The gene order and composition of A. monticolus was similar to that of most other vertebrates. The descending order of the base composition on heavy strand was 31.4% A, 28.2% C, 24.5% T, 15.8% G, with a relatively lower level of G and a slight AT bias of 55.9%. The codon usage followed the typical vertebrate mitochondrial pattern (ATG or GTG for start codon and TAA or TAG for stop codon). There are 7 regions of gene overlap totaling 23 bp and 13 intergenic spacer regions totaling 69 bp.
利用长PCR反应测定了中国特有的山瑞光唇鱼的完整线粒体基因组序列。该基因组长度为16599 bp，包括13个典型的脊椎动物蛋白质编码基因、22个转运RNA基因、2个核糖体RNA基因和一个控制区。除了8个tRNA和ND6基因外，所有其他线粒体基因都由重链编码。山瑞光唇鱼的基因顺序和组成与大多数其他脊椎动物相似。重链上碱基组成的降序排列为：A占31.4%、C占28.2%、T占24.5%、G占15.8%，G含量相对较低，AT偏倚率为55.9%。密码子使用遵循典型的脊椎动物线粒体模式（起始密码子为ATG或GTG，终止密码子为TAA或TAG）。共有7个基因重叠区，总长23 bp，13个基因间隔区，总长69 bp。
