Centre de Recherches sur les Macromolécules Végétales, CERMAV-CNRS (Affiliated with Université Joseph Fourier, and member of the Institut de Chimie Moléculaire de Grenoble), Grenoble, France.
Biomacromolecules. 2012 Apr 9;13(4):1129-35. doi: 10.1021/bm3000138. Epub 2012 Mar 23.
The preparation of biocompatible nanocarriers that have potential applications in the cosmetic and health industries is highly desired. The self-assembly of amphiphilic block copolymers displaying biosourced polysaccharides at the surface is one of the most promising approaches. In the continuity of our works related to the preparation of "hybrid" amphiphilic oligosaccharide-based block copolymers, we present here the design of a new generation of self-assembled nanoparticles composed entirely of oligosaccharide-based amphiphilic block co-oligomers (BCO). These systems are defined by a covalent linkage of the two saccharidic blocks through their reducing end units, resulting in a sweet "head-to-head" connection. As an example, we have prepared and studied a BCO in which the hydrophilic part is composed of a free maltoheptaosyl derivative clicked to a hydrophobic part composed of a peracetylated maltoheptaosyl derivative. This amphiphilic BCO self-assembles to form spherical micelles in water with an average diameter of 30 nm. The efficient enzymatic hydrolysis of the maltoheptaose that constitutes the shell of the micelles was followed by light scattering and colorimetric methods.
制备具有在化妆品和健康产业中有潜在应用的生物相容性纳米载体是非常需要的。具有生物源多糖表面展示的两亲性嵌段共聚物的自组装是最有前途的方法之一。在我们与“杂化”两亲性低聚糖基嵌段共聚物的制备相关的工作的延续中，我们在这里提出了完全由低聚糖基两亲性嵌段共聚寡聚物（BCO）组成的新一代自组装纳米粒子的设计。这些系统通过其还原端单元通过共价键连接两个糖基块，从而形成甜“头对头”连接。例如，我们已经制备并研究了一种 BCO，其中亲水部分由游离的麦芽七糖衍生物点击到由乙酰化麦芽七糖衍生物组成的疏水部分组成。这种两亲性 BCO 在水中自组装形成平均直径为 30nm 的球形胶束。通过光散射和比色法跟踪构成胶束壳的麦芽七糖的有效酶水解。
