Department of Ocular Biology & Therapeutics, UCL Institute of Ophthalmology, London, UK.
Regen Med. 2012 Mar;7(2):167-78. doi: 10.2217/rme.11.117.
To investigate the optimum conditions for the differentiation of a mouse embryonic stem cell line towards corneal epithelial cell fate.
MATERIALS & METHODS: The effect of conditioned media from both metabolically active (to produce lineage A) and growth-arrested limbal fibroblasts (lineage G) were compared with basal media (lineage N) in terms of morphology and marker expression, assessed by immunocytochemistry and reverse transcription PCR. Cultures were transplanted into a porcine ex vivo model to investigate their ability for wound healing and cornea repair.
Lineage N exhibited cobblestone morphology and expressed CK12 and p63α, while OCT4 and SSEA1 were downregulated. Post-transplantation, these cells were able to multilayer and heal after wounding while maintaining marker expression.
Lineages with corneal epithelial-like characteristics, which are derived from embryonic stem cells, have potential for use in the study of corneal wound healing and therapy.
研究小鼠胚胎干细胞向角膜上皮细胞命运分化的最佳条件。
通过免疫细胞化学和逆转录 PCR 分析，比较代谢活跃（产生谱系 A）和生长停滞的角膜缘成纤维细胞（谱系 G）的条件培养基与基础培养基（谱系 N）在形态和标志物表达方面的差异。将培养物移植到猪体外模型中，以研究其在伤口愈合和角膜修复方面的能力。
谱系 N 呈现鹅卵石样形态，表达 CK12 和 p63α，同时 OCT4 和 SSEA1 下调。移植后，这些细胞能够多层化，并在伤口愈合后保持标志物表达。
来源于胚胎干细胞的具有角膜上皮样特征的谱系细胞具有用于研究角膜伤口愈合和治疗的潜力。
