Tanumihardjo S A, Yuniar Y, Permaesih D, Sulaiman Z, Karyadi D, Olson J A
Department of Biochemistry and Biophysics, Iowa State University, Ames 50011.
Am J Clin Nutr. 1990 Dec;52(6):1068-72. doi: 10.1093/ajcn/52.6.1068.
The modified relative-dose-response (MRDR) assay has been validated in rats as a function of vitamin A status and tested in a group of American children. In this study the MRDR assay was applied to West Javan children who are at risk of being vitamin A deficient. Of 86 children enrolled, 75 were tested. In a time-course study involving 22 children aged 3.7-5.3 y, blood samples were taken at different times after doses of 0.35 mumol 3,4-didehydroretinyl acetate/kg body wt. Generally, the ratio of dehydroretinol to retinol (DR-R ratio) peaked between 4 and 8 h. Thereafter, in a survey of 53 children aged 0.6-4.8 y, single blood samples were drawn 5 h after the dehydroretinyl acetate dose. The DR-R ratio ranged from 0.0028 to 0.169. With a DR-R ratio of 0.03 as the cutoff value, 62% of the children were judged to be of marginal vitamin A status.
改良相对剂量反应（MRDR）试验已在大鼠中作为维生素A状态的一项功能得到验证，并在美国一组儿童中进行了测试。在本研究中，MRDR试验应用于有维生素A缺乏风险的西爪哇儿童。在登记的86名儿童中，75名接受了测试。在一项涉及22名年龄在3.7至5.3岁儿童的时间进程研究中，在给予0.35 μmol 3,4-去氢视黄酯乙酸酯/千克体重的剂量后，在不同时间采集血样。一般来说，脱氢视黄醇与视黄醇的比率（DR-R比率）在4至8小时之间达到峰值。此后，在一项对53名年龄在0.6至4.8岁儿童的调查中，在给予乙酸脱氢视黄酯剂量5小时后采集单个血样。DR-R比率范围为0.0028至0.169。以DR-R比率0.03作为临界值，62%的儿童被判定为维生素A边缘状态。
