Division of Blood Purification, Kanazawa University Hospital, 13-1 Takara-machi, Kanazawa 920-8641, Japan.
Clin Exp Nephrol. 2012 Oct;16(5):679-89. doi: 10.1007/s10157-012-0614-6. Epub 2012 Mar 8.
Acute kidney injury (AKI) is a critical condition for kidney and other remote organs, including the lung. However, available treatments for AKI are limited. In this study, we explored the effect of adipose-derived mesenchymal cells on a mouse model of AKI.
Adipose-derived mesenchymal cells were isolated from mouse subcutaneous and peritoneal adipose tissue by digestion with collagenase type I. The left renal artery and vein of C57BL/6 mice were clamped for 45 min to induce ischemia and were injected with the adipose-derived mesenchymal cells [1 × 10(5) cells/0.2 ml phosphate-buffered saline (PBS)] or 0.2 ml PBS via the tail vein on days 0, 1, and 2.
The adipose-derived mesenchymal cells had stem-cell surface markers and multilineage differentiating potentials. Administered adipose-derived mesenchymal cells homed primarily into lung. Interestingly, repeated administration of adipose-derived mesenchymal cells reduced acute tubular necrosis and interstitial macrophage infiltration in the injured kidney, accompanied with reduced cytokine and chemokine expression.
Adipose-derived mesenchymal cells can be used as cell-based therapy for ischemic kidney injury.
急性肾损伤（AKI）是肾脏和其他远程器官（包括肺）的危急状况。然而，现有的 AKI 治疗方法有限。在这项研究中，我们探讨了脂肪来源间充质细胞对 AKI 小鼠模型的影响。
通过胶原酶 I 消化从小鼠皮下和腹膜脂肪组织中分离脂肪来源间充质细胞。夹闭 C57BL/6 小鼠的左肾动静脉 45 分钟以诱导缺血，并通过尾静脉在第 0、1 和 2 天注射脂肪来源间充质细胞[1×10(5)个细胞/0.2 ml 磷酸盐缓冲盐水（PBS）]或 0.2 ml PBS。
脂肪来源间充质细胞具有干细胞表面标志物和多系分化潜能。给予的脂肪来源间充质细胞主要归巢到肺。有趣的是，重复给予脂肪来源间充质细胞可减少损伤肾脏的急性肾小管坏死和间质巨噬细胞浸润，并伴有细胞因子和趋化因子表达减少。
脂肪来源间充质细胞可用作缺血性肾损伤的基于细胞的治疗方法。
