Department of Neurosurgery, Faculty of Medicine, University of Ondokuz Mayis, 55139, Samsun, Turkey.
Neurochem Res. 2012 Jul;37(7):1465-74. doi: 10.1007/s11064-012-0737-x. Epub 2012 Mar 8.
The aim of this study was to investigate the interaction between nitric oxide (NO) and acetylcholine (ACh) in penicillin-induced experimental epilepsy. Adult male Wistar rats weighing 220 ± 35 g were used in the experiments. The epileptiform activity was induced by microinjection of penicillin (200 IU/1 μl) into the left sensorymotor cortex. Electrocorticogram was recorded by using Ag/AgCl ball electrodes. Sodium nitroprusside (SNP), a NO donor, given intracortically 30 min after penicillin significantly reduced the spike frequency whereas ACh increased the epileptiform activity for 5 min. Atropine, an antagonist for muscarinic receptors, was given intracortically 30 min after penicillin and did not significantly affect epileptiform activity for 30 min. SNP given after atropine significantly suppressed the epileptiform activity. ACh given 10 min after Nω-nitro-L: -arginine methyl ester (L-NAME), a nonspecific nitric oxide synthase inhibitor, did not have a significant effect on spike frequency. When ACh and SNP were administered together, penicillin induced epileptiform activity and spike frequency were significantly suppressed from the 10th minute onwards. It can be concluded that ACh increases the penicillin-induced epileptiform activity while co-administration of ACh and SNP produces a potent anticonvulsant effect as compared to SNP alone.
本研究旨在探讨一氧化氮（NO）和乙酰胆碱（ACh）在青霉素诱导的实验性癫痫中的相互作用。实验采用成年雄性 Wistar 大鼠，体重 220±35g。将青霉素（200IU/1μl）注入左侧感觉运动皮层诱导癫痫样活动。采用 Ag/AgCl 球电极记录皮质电图。NO 供体硝普钠（SNP）在青霉素后 30 分钟内皮层内给药可显著降低尖峰频率，而乙酰胆碱（ACh）可使癫痫样活动增加 5 分钟。阿托品，一种毒蕈碱受体拮抗剂，在青霉素后 30 分钟内皮层内给药，在 30 分钟内对癫痫样活动无明显影响。SNP 在给予阿托品后可显著抑制癫痫样活动。在非特异性一氧化氮合酶抑制剂 Nω-硝基-L：-精氨酸甲酯（L-NAME）后 10 分钟给予 ACh，对尖峰频率没有明显影响。当 ACh 和 SNP 一起给药时，从第 10 分钟开始，青霉素诱导的癫痫样活动和尖峰频率明显受到抑制。可以得出结论，ACh 增加了青霉素诱导的癫痫样活动，而 ACh 和 SNP 的联合给药与单独给予 SNP 相比产生了更强的抗惊厥作用。
