Marangoz C, Ayyildiz M, Ağar E
Department of Physiology, Faculty of Medicine, University of Ondokuz Mayis, Samsun-Turkey.
Neuroreport. 1994 Dec 20;5(18):2454-6. doi: 10.1097/00001756-199412000-00012.
The effect of sodium nitroprusside (SNP) on epileptiform activity elicited by administration of penicillin (500 units) into the somatomotor cortex was studied in anaesthetized rats. No epileptiform activity was observed after intracortical microinjection of SNP (5 and 20 nM). Microinjection of penicillin into the somatomotor cortex induced epileptiform activity in electrocorticograms (ECoG). Epileptiform discharges elicited by penicillin were significantly decreased by SNP. The effect of SNP appeared within 1 min of application and lasted for 2-5 min. The inhibitory effect of SNP on epileptiform activity could be prevented by pretreatment with methylene blue (20 nM), a soluble guanylate cyclase inhibitor. Prior injection of haemoglobin (5 microliter), a nitric oxide (NO) scavenger, prevented the anticonvulsant effect of SNP. These results suggest that NO may be an endogenous anticonvulsant substance.
在麻醉大鼠中，研究了硝普钠（SNP）对向躯体运动皮层注射青霉素（500单位）所引发的癫痫样活动的影响。皮层内微量注射SNP（5和20 nM）后未观察到癫痫样活动。向躯体运动皮层微量注射青霉素可在脑电图（ECoG）中诱发癫痫样活动。SNP可显著降低青霉素引发的癫痫样放电。SNP的作用在应用后1分钟内出现，并持续2 - 5分钟。SNP对癫痫样活动的抑制作用可被可溶性鸟苷酸环化酶抑制剂亚甲蓝（20 nM）预处理所阻断。预先注射一氧化氮（NO）清除剂血红蛋白（5微升）可阻断SNP的抗惊厥作用。这些结果表明，NO可能是一种内源性抗惊厥物质。
