Department of Biological Science and Technology, Tokyo University of Science, Noda, Chiba, Japan.
Adv Exp Med Biol. 2012;727:37-46. doi: 10.1007/978-1-4614-0899-4_3.
Notch is a single-pass transmembrane receptor that mediates the local cell-cell interactions necessary for many cell-fate decisions. The extra cellular domain of Notch contains a tandem array of epidermal growth factor-like (EGF-like) repeats. Some of these EGF-like repeats are O-fucosylated by protein O-fucosyltransferase 1 (O-fut1), which is essential for Notch signaling in Drosophila and mouse. This O-fucose is further modified by Fringe, a GlcNAc transferase and other glycosyltransferases (O-fut1 in Drosophila and Pofut1 in mouse), to form an O-linked tetrasaccharide, which modulates Notch's selective binding to its ligands.
Notch 是一种单次跨膜受体，介导许多细胞命运决定所必需的局部细胞-细胞相互作用。Notch 的细胞外结构域包含串联排列的表皮生长因子样（EGF 样）重复序列。这些 EGF 样重复序列中的一些被蛋白质 O-岩藻糖基转移酶 1（O-fut1）O-岩藻糖基化，这对于果蝇和小鼠中的 Notch 信号转导至关重要。这种 O-岩藻糖基进一步被 Fringe（一种 GlcNAc 转移酶和其他糖基转移酶）修饰，形成 O-连接的四糖，调节 Notch 与其配体的选择性结合。
