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使用非晶硅碳(a-SiC:H)多层换能器检测活性氰化物与黄色荧光团之间荧光的变化。

Detection of change in fluorescence between reactive cyan and the yellow fluorophores using a-SiC:H multilayer transducers.

作者信息

Vieira M, Costa J, Vieira M A, Louro P, Fernandes M, Fantoni A

机构信息

Electronics Telecommunication and Computer Department ISEL, R. Conselheiro Emídio Navarro, 1949-014 Lisboa, Portugal.

出版信息

J Nanosci Nanotechnol. 2011 Oct;11(10):8657-62. doi: 10.1166/jnn.2011.3476.

DOI:10.1166/jnn.2011.3476
PMID:22400239
Abstract

Optical colour sensors based on multilayered a-SiC:H heterostructures can act as voltage controlled optical filters in the visible range. In this article we investigate the application of these structures for Fluorescence Resonance Energy Transfer (FRET) detection, The characteristics of a-SiC:H multilayered structure are studied both theoretically and experimentally in several wavelengths corresponding to different fluorophores. The tunable optical p-i'(a-SiC:H)-n/p-i(a-Si:H)-n heterostructures were produced by PECVD and tested for a proper fine tuning in the violet, cyan and yellow wavelengths. The devices were characterized through transmittance and spectral response measurements, under different electrical bias and frequencies. Violet, cyan and yellow signals were applied in simultaneous and results have shown that they can be recovered under suitable applied bias. A theoretical analysis supported by numerical simulation is presented.

摘要

基于多层非晶硅碳氢(a-SiC:H)异质结构的光学颜色传感器可作为可见光范围内的电压控制光学滤波器。在本文中,我们研究了这些结构在荧光共振能量转移(FRET)检测中的应用。在对应于不同荧光团的几个波长下,对a-SiC:H多层结构的特性进行了理论和实验研究。通过等离子体增强化学气相沉积(PECVD)制备了可调谐光学p-i'(a-SiC:H)-n/p-i(a-Si:H)-n异质结构,并在紫光、青光和黄光波长下进行了适当的微调测试。在不同的电偏压和频率下,通过透射率和光谱响应测量对器件进行了表征。同时施加了紫光、青光和黄光信号,结果表明在合适的外加偏压下它们可以被恢复。本文还给出了由数值模拟支持的理论分析。

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