Baccar Z M, Hidouri S, Errachid A, Sanchez O Ruiz
nanoBioengineering Group, National Institute of Research and Physicochemical Analysis (INRAP), Biotechpôle de Sidi Thabet, 2020NSidi Thabet, Tunisia.
J Nanosci Nanotechnol. 2011 Oct;11(10):8798-803. doi: 10.1166/jnn.2011.3461.
In this work, we present the development of a hybrid biomembrane based on the immobilization of diamine oxidase (DAO) into LDH thin films for histamine detection. The LDHs preselected as host matrixes are: hydrotalcites (Mg2Al(CO3)0.5(OH)6), lowaite (Mg4Fe(OH)10Cl) and hydrocalumite (Ca2Al(OH)6Cl). The immobilized probes were characterized by atomic force microscopy (AFM) and attenuated total reflection infrared spectroscopy (IR-ATR mode). The analysis of these results shows that the immobilization of DAO occurs with all type of selected LDH and is stable after a 7 day-immersion in phosphate buffer solution. The LDH incorporating magnesium or calcium divalent cations present high-quality surface topology for DAO immobilization and the ability to keep the enzyme in a well conformation for biogenic amines catabolism and histamine detection.
在这项工作中，我们展示了一种基于将二胺氧化酶（DAO）固定到层状双氢氧化物（LDH）薄膜中用于组胺检测的混合生物膜的开发。预先选定作为主体基质的层状双氢氧化物有：水滑石（Mg2Al(CO3)0.5(OH)6）、水铁矿（Mg4Fe(OH)10Cl）和水钙铝榴石（Ca2Al(OH)6Cl）。通过原子力显微镜（AFM）和衰减全反射红外光谱（IR-ATR模式）对固定化探针进行了表征。对这些结果的分析表明，DAO能固定在所有选定类型的层状双氢氧化物上，并且在磷酸盐缓冲溶液中浸泡7天后仍保持稳定。含有镁或钙二价阳离子的层状双氢氧化物为DAO固定提供了高质量的表面拓扑结构，并且能够使酶保持良好构象以进行生物胺分解代谢和组胺检测。
