IEEE Trans Nanobioscience. 2017 Dec;16(8):650-655. doi: 10.1109/TNB.2017.2736781. Epub 2017 Aug 7.
Hybrid thin films based on Hydrocalumite (CaAlCl layered double hydroxide LDH) and tyrosinaseenzyme have been used for the elaboration of a high sensitive amperometric biosensor detecting polyphenols extracted from green tea. Structural properties of LDH nanomaterials were characterized by X-ray powder diffraction and Infra-Red spectroscopy, confirming its crystalline phase and chemical composition. CaAlCl-LDHs-thin films were deposited by spin-coating, and studied by atomic force microscopy to obtain information about the surface morphology of this host matrix before and after enzyme's immobilization. Electrochemical study using cyclic voltammetry and chronoamperometry shows good performances of the built-in biosensor with a high sensitivity for polyphenols concentrations ranging from 24 pM to and a limit of detection of 1.2 pM.
基于水滑石（CaAlCl 层状双氢氧化物 LDH）和酪氨酸酶的混合薄膜已被用于研制一种高灵敏度的电流型生物传感器，用于检测从绿茶中提取的多酚。通过 X 射线粉末衍射和红外光谱对 LDH 纳米材料的结构性质进行了表征，证实了其晶相和化学成分。通过旋涂法沉积 CaAlCl-LDHs 薄膜，并通过原子力显微镜对其进行研究，以获得酶固定化前后这种主体基质的表面形貌信息。使用循环伏安法和计时电流法进行电化学研究表明，内置生物传感器具有良好的性能，对多酚浓度具有高灵敏度，范围从 24 pM 到 ，检测限为 1.2 pM。
