Lazarus S C, Chesrown S E, Frey M J, Reed B R, Mjörndal T O, Gold W M
J Appl Physiol Respir Environ Exerc Physiol. 1979 May;46(5):919-26. doi: 10.1152/jappl.1979.46.5.919.
We studied the effects of Ascaris suum antigen (iv) in 32 natively allergic, anesthetized dogs. After thoracotomy, bilateral cervical vagotomy, and propranolol, samples of peripheral lung frozen in situ were obtained for measurement of cyclic nucleotides and histamine. Following Ascaris, lung histamine decreased 20.4 +/- 3.7% (mean +/- SE), cAMP increased 391 +/- 122%, and cGMP increased 110 +/- 20% with increased plasma histamine and physiological changes of anaphylaxis. No significant changes occurred in 10 control dogs. Release of histamine, reflecting immunological degranulation of mast cells, correlated closely with the physiological effects of anaphylaxis. beta-Adrenergic stimulation with isoproterenol prevented these physiological effects of anaphylaxis in three dogs studied. Furthermore, the level of cAMP induced in lung tissue by beta-adrenergic stimulation in these dogs correlated with the degree of inhibition of immunologically induced histamine release. These results illustrate the suitability of this experimental preparation to study the biochemical and physiological mechanisms of anaphylaxis in vivo.
我们研究了猪蛔虫抗原（静脉注射）对32只天然过敏的麻醉犬的影响。开胸、双侧颈迷走神经切断和给予普萘洛尔后，获取原位冷冻的外周肺组织样本，用于测量环核苷酸和组胺。注射猪蛔虫抗原后，肺组织组胺减少20.4±3.7%（平均值±标准误），环磷酸腺苷（cAMP）增加391±122%，环磷酸鸟苷（cGMP）增加110±20%，同时血浆组胺增加，出现过敏反应的生理变化。10只对照犬未出现显著变化。组胺的释放反映了肥大细胞的免疫脱颗粒，与过敏反应的生理效应密切相关。在研究的3只犬中，用异丙肾上腺素进行β-肾上腺素能刺激可预防过敏反应的这些生理效应。此外，这些犬肺组织中β-肾上腺素能刺激诱导的cAMP水平与免疫诱导的组胺释放抑制程度相关。这些结果表明该实验制剂适用于研究体内过敏反应的生化和生理机制。
