Takahashi I, Kern M K, Dodds W J, Hogan W J, Layman R D, Ammon H V
Department of Radiology, Medical College of Wisconsin, Milwaukee 53226.
Am J Physiol. 1990 Nov;259(5 Pt 1):G745-52. doi: 10.1152/ajpgi.1990.259.5.G745.
In conscious opossums, we evaluated the relationship between hepatic bile flow and the intestinal motor function during fasting as well as after feeding. In six opossums, bipolar electrodes were implanted from the gastric antrum to the terminal ileum. After cholecystectomy, the common duct was ligated, and a catheter was tied into the proximal common duct for collecting hepatic bile. During subsequent studies, hepatic bile flow was measured, and bile was returned to the duodenum through an externalized duodenal catheter. Cyclic increases in bile flow during fasting did not show a close correlate with the duodenal migratory motor complex (MMC) cycle. Rather, bile flow showed peak values [0.11 +/- 0.02 (SE) ml/min] when phase III MMC activity reached the midileum. Hepatic bile flow correlated closely with the amount of bile acid secreted by the liver. When the bile acid pool was depleted by diverting bile from the intestine, hepatic secretion of bile fell to uniformly low values of approximately 0.04 ml/min that did not show cyclic variation. Hepatic bile flow after feeding increased to a maximal value of 0.12 +/- 0.01 ml/min at 90 min. We conclude that increases in hepatic bile flow during fasting and after meals are determined mainly by variations in intestinal motor activity that alter small bowel transit and thereby affect the enterohepatic circulation of bile acids.
在清醒的负鼠中，我们评估了禁食期间以及进食后肝胆汁流量与肠道运动功能之间的关系。在六只负鼠中，将双极电极从胃窦植入到回肠末端。在胆囊切除术后，结扎胆总管，并将一根导管系于胆总管近端以收集肝胆汁。在随后的研究中，测量肝胆汁流量，并通过外置的十二指肠导管将胆汁回输至十二指肠。禁食期间胆汁流量的周期性增加与十二指肠移行性运动复合波（MMC）周期并无密切关联。相反，当MMC活动的第三相到达回肠中部时，胆汁流量出现峰值[0.11±0.02（标准误）ml/分钟]。肝胆汁流量与肝脏分泌的胆汁酸量密切相关。当通过将胆汁从肠道分流使胆汁酸池耗竭时，肝胆汁分泌降至约0.04 ml/分钟的统一低水平，且未显示出周期性变化。进食后肝胆汁流量在90分钟时增加至最大值0.12±0.01 ml/分钟。我们得出结论，禁食期间和进食后肝胆汁流量的增加主要由肠道运动活动的变化所决定，这些变化改变了小肠转运，从而影响胆汁酸的肠肝循环。
