Department of Clinical Laboratory, The Second Xiangya Hospital, Central South University, Changsha, 410011 Hunan, PR China.
Clin Chim Acta. 2012 Jun 14;413(11-12):973-7. doi: 10.1016/j.cca.2012.02.019. Epub 2012 Mar 3.
Tyrosine (Tyr), Tryptophan (Trp) and 5-hydroxytryptamine (5-HT) are important amino acids in vivo and have been hypothesized to be involved in many mental disorders. We developed a rapid and sensitive HPLC method for simultaneous measurement of serum Tyr, Trp and 5-HT and explored the clinical significances of Tyr, Trp and 5-HT and the 5-HT/Trp ratio for patients with major depressive disorder (MDD) disease.
Serum samples were deproteinized by 5% perchloric acid and separated on an Atlantis C18 column (4.6 × 150 mm, 5 μm) with the mobile phase consisting of 0.1 mol/l KH(2)PO(4) and methanol (85:15, V/V).The eluates were monitored by the fluorescence detection with programmed wavelength.
Analysis was achieved in <12.0 min. The limits of quantification were 0.014, 0.005, and 0.024 μmol/l for Tyr, Trp and 5-HT, respectively. Reproducibility and recovery were satisfactory. Tyr, Trp and 5-HT and the 5-HT/Trp ratio were significantly decreased in patients with MDD.
In diseases, like MDD, Tyr, Trp and 5-HT play an important role. This method can potentially be applied as prognostic or diagnostic tool or even to follow the evolution of the illness or of the treatment.
酪氨酸（Tyr）、色氨酸（Trp）和 5-羟色胺（5-HT）是体内重要的氨基酸，据推测它们与许多精神疾病有关。我们开发了一种快速灵敏的 HPLC 方法，用于同时测定血清 Tyr、Trp 和 5-HT，并探讨了 Tyr、Trp 和 5-HT 及 5-HT/Trp 比值对重度抑郁症（MDD）患者的临床意义。
血清样品用 5%高氯酸沉淀，用流动相（0.1 mol/L KH（2）PO（4）和甲醇，85:15，V/V）在 Atlantis C18 柱（4.6×150mm，5μm）上分离。洗脱液用程序波长荧光检测法监测。
分析时间小于 12.0 分钟。Tyr、Trp 和 5-HT 的定量下限分别为 0.014、0.005 和 0.024 μmol/L。重现性和回收率良好。MDD 患者的 Tyr、Trp 和 5-HT 及 5-HT/Trp 比值明显降低。
在 MDD 等疾病中，Tyr、Trp 和 5-HT 发挥着重要作用。该方法有望成为预后或诊断工具，甚至可用于监测疾病或治疗的进展。
