Manning R D
Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson 39216-4505.
Am J Physiol. 1990 Nov;259(5 Pt 2):H1317-24. doi: 10.1152/ajpheart.1990.259.5.H1317.
Studies were performed in 14 conscious, anephric dogs to clarify the role of blood volume in the genesis of hypertension. The dogs were splenectomized and had plasma protein concentration (PPC) reduced to 2.7 g/dl by daily plasmapheresis for 9 days. This hypoproteinemia resulted in a 20% decrease in both blood volume and mean arterial pressure. On the 10th day the dogs were nephrectomized. On the 11th day after a 3-h control period with plasmapheresis, lactated Ringer equivalent to 10 or 20% of body weight was intravenously infused. By 25 h postinfusion blood volume had not increased, and the dogs were still hypotensive. At 25 h plasma protein mass was returned to normal by intravenous infusion of autologous plasma, the average blood volume of the three low PPC groups increased approximately 50%, and the arterial pressure increased greater than 60%. The decrease in PPC shifted the regression of blood volume on sodium space down the blood volume axis. In conclusion, the dependence of arterial pressure on blood volume was demonstrated by the decrease in both blood volume and arterial pressure after PPC reduction, the constancy of blood volume and pressure during Ringer infusion, and the increase in both volume and pressure after plasma infusion.
对14只清醒的无肾犬进行了研究，以阐明血容量在高血压发生过程中的作用。这些犬接受了脾切除术，并通过每日进行9天的血浆置换使血浆蛋白浓度（PPC）降至2.7 g/dl。这种低蛋白血症导致血容量和平均动脉压均降低20%。在第10天，对这些犬实施了肾切除术。在第11天，经过3小时的血浆置换对照期后，静脉输注相当于体重10%或20%的乳酸林格液。输注后25小时，血容量未增加，犬仍处于低血压状态。在25小时时，通过静脉输注自体血浆使血浆蛋白量恢复正常，三个低PPC组的平均血容量增加了约50%，动脉压升高超过60%。PPC的降低使血容量对钠间隙的回归线沿血容量轴下移。总之，PPC降低后血容量和动脉压均下降、林格液输注期间血容量和血压保持恒定以及血浆输注后血容量和血压均升高，证明了动脉压对血容量的依赖性。
