Chemical Pathology Department, Pathology Queensland, Royal Brisbane and Women's Hospital, Herston Road, Brisbane, Queensland 4029, Australia.
Ann Clin Biochem. 2012 May;49(Pt 3):242-56. doi: 10.1258/acb.2011.011158. Epub 2012 Mar 8.
Although protein electrophoresis of serum (SPEP) and urine (UPEP) specimens is a well-established laboratory technique, the reporting of results using this important method varies considerably between laboratories. The Australasian Association of Clinical Biochemists recognized a need to adopt a standardized approach to reporting SPEP and UPEP by clinical laboratories.
A Working Party considered available data including published literature and clinical studies, together with expert opinion in order to establish optimal reporting practices. A position paper was produced, which was subsequently revised through a consensus process involving scientists and pathologists with expertise in the field throughout Australia and New Zealand.
Recommendations for standardized reporting of protein electrophoresis have been produced. These cover analytical requirements: detection systems; serum protein and albumin quantification; fractionation into alpha-1, alpha-2, beta and gamma fractions; paraprotein quantification; urine Bence Jones protein quantification; paraprotein characterization; and laboratory performance, expertise and staffing. The recommendations also include general interpretive commenting and commenting for specimens with paraproteins and small bands together with illustrative examples of reports.
Recommendations are provided for standardized reporting of protein electrophoresis in Australia and New Zealand. It is expected that such standardized reporting formats will reduce both variation between laboratories and the risk of misinterpretation of results.
尽管血清蛋白电泳（SPEP）和尿液蛋白电泳（UPEP）标本的蛋白电泳是一种成熟的实验室技术，但不同实验室之间使用这种重要方法报告结果的方式差异很大。澳大利亚临床生物化学家协会认识到有必要采用标准化方法来报告临床实验室的 SPEP 和 UPEP。
一个工作组考虑了可用的数据，包括已发表的文献和临床研究，以及该领域专家的意见，以确定最佳的报告实践。提出了一份立场文件，随后通过一个涉及澳大利亚和新西兰具有该领域专业知识的科学家和病理学家的共识过程进行了修订。
提出了标准化报告蛋白电泳的建议。这些建议涵盖了分析要求：检测系统；血清蛋白和白蛋白定量；分为α-1、α-2、β和γ 馏分；副蛋白定量；尿 Bence Jones 蛋白定量；副蛋白特征；以及实验室性能、专业知识和人员配备。建议还包括一般解释性评论和有副蛋白和小条带标本的评论，以及报告的示例。
为澳大利亚和新西兰的蛋白电泳标准化报告提供了建议。预计这种标准化报告格式将减少实验室之间的差异和结果误读的风险。
