Department of Endodontics and Molecular Microbiology Laboratory, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, Rio de Janeiro, Brazil.
J Clin Microbiol. 2012 May;50(5):1721-4. doi: 10.1128/JCM.00531-12. Epub 2012 Mar 7.
This study evaluated the microbiota of root canals undergoing retreatment. The most prevalent taxa detected by checkerboard included Propionibacterium species, Fusobacterium nucleatum, streptococci, and Pseudoramibacter alactolyticus. Quantitative real-time PCR detected Enterococcus faecalis and streptococci in 38% and 41% of the cases, comprising 9.76% and 65.78% of the total bacterial counts, respectively. The findings call into question the status of E. faecalis as the main pathogen and suggest that other species can be candidate pathogens associated with persistent/secondary endodontic infections.
本研究评估了进行再治疗的根管内微生物群落。通过 checkerboard 检测到的最常见的类群包括丙酸杆菌属、核梭杆菌、链球菌和缓症拟杆菌。定量实时 PCR 检测到粪肠球菌和链球菌在 38%和 41%的病例中，分别占总细菌计数的 9.76%和 65.78%。这些发现对粪肠球菌作为主要病原体的地位提出了质疑，并表明其他物种可能是与持续性/继发性根管感染相关的候选病原体。
