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集胞藻 PCC6803 的 rnb 基因编码一种 RNA 水解酶，具有 RNase II 而不是 RNase R 的酶学特性。

The rnb gene of Synechocystis PCC6803 encodes a RNA hydrolase displaying RNase II and not RNase R enzymatic properties.

机构信息

Instituto de Tecnologia Química e Biológica/Universidade Nova de Lisboa, Oeiras, Portugal.

出版信息

PLoS One. 2012;7(3):e32690. doi: 10.1371/journal.pone.0032690. Epub 2012 Mar 5.

DOI:10.1371/journal.pone.0032690
PMID:22403697
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3293843/
Abstract

Cyanobacteria are photosynthetic prokaryotic organisms that share characteristics with bacteria and chloroplasts regarding mRNA degradation. Synechocystis sp. PCC6803 is a model organism for cyanobacteria, but not much is known about the mechanism of RNA degradation. Only one member of the RNase II-family is present in the genome of Synechocystis sp PCC6803. This protein was shown to be essential for its viability, which indicates that it may have a crucial role in the metabolism of Synechocystis RNA. The aim of this work was to characterize the activity of the RNase II/R homologue present in Synechocystis sp. PCC6803. The results showed that as expected, it displayed hydrolytic activity and released nucleoside monophosphates. When compared to two E. coli counterparts, the activity assays showed that the Synechocystis protein displays RNase II, and not RNase R characteristics. This is the first reported case where when only one member of the RNase II/R family exists it displays RNase II and not RNase R characteristics.

摘要

蓝藻是光合原核生物，在 mRNA 降解方面与细菌和叶绿体具有共同特征。集胞藻 PCC6803 是蓝藻的模式生物，但关于 RNA 降解的机制知之甚少。集胞藻 PCC6803 的基因组中仅存在一个 RNase II 家族成员。该蛋白被证明对其生存能力是必需的，这表明它可能在集胞藻 RNA 的代谢中发挥关键作用。本工作旨在表征集胞藻 PCC6803 中存在的 RNase II/R 同源物的活性。结果表明，正如预期的那样，它显示出水解活性并释放核苷单磷酸。与两种大肠杆菌对应物相比，活性测定表明集胞藻蛋白显示 RNase II 而不是 RNase R 特征。这是首例报道的当 RNase II/R 家族中仅存在一个成员时，它显示 RNase II 而不是 RNase R 特征的情况。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd34/3293843/8097cb04f005/pone.0032690.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd34/3293843/48cfaa72c912/pone.0032690.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd34/3293843/a459ae5a3e6e/pone.0032690.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd34/3293843/04b71fbe4f0f/pone.0032690.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd34/3293843/68329f1e7c98/pone.0032690.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd34/3293843/5c539205ecd1/pone.0032690.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd34/3293843/4ac9e964a74c/pone.0032690.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd34/3293843/628b2f515890/pone.0032690.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd34/3293843/b392aac51136/pone.0032690.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd34/3293843/b22f9ab8ed3e/pone.0032690.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd34/3293843/8097cb04f005/pone.0032690.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd34/3293843/48cfaa72c912/pone.0032690.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd34/3293843/a459ae5a3e6e/pone.0032690.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd34/3293843/04b71fbe4f0f/pone.0032690.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd34/3293843/68329f1e7c98/pone.0032690.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd34/3293843/5c539205ecd1/pone.0032690.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd34/3293843/4ac9e964a74c/pone.0032690.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd34/3293843/628b2f515890/pone.0032690.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd34/3293843/b392aac51136/pone.0032690.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd34/3293843/b22f9ab8ed3e/pone.0032690.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd34/3293843/8097cb04f005/pone.0032690.g010.jpg

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