Center for Plant Cell Biology, Institute for Integrative Genome Biology, Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA.
Annu Rev Plant Biol. 2012;63:261-82. doi: 10.1146/annurev-arplant-042811-105456. Epub 2012 Jan 30.
Since the introduction of chemical genomics to plant biology as a tool for basic research, the field has advanced significantly. There are now examples of important basic discoveries that demonstrate the power and untapped potential of this approach. Given the combination of protein and small-molecule complexity, new phenotypes can be described through the perturbation of cellular functions that can be linked to growth and developmental phenotypes. There are now clear examples of overcoming functional redundancy in plants to dissect molecular mechanisms or critical pathways such as hormone signaling and dynamic intracellular processes. Owing to ongoing advances, including more sophisticated high-content screening and rapid approaches for target identification, the field is beginning to move forward. However, there are also challenges to improve automation, imaging, and analysis and provide chemical biology resources to the broader plant biology community.
自从化学基因组学作为一种基础研究工具被引入植物生物学领域以来，该领域已经取得了显著的进展。现在已经有一些重要的基础发现的例子，证明了这种方法的强大威力和尚未开发的潜力。鉴于蛋白质和小分子的复杂性，通过干扰与生长和发育表型相关的细胞功能，可以描述新的表型。现在已经有明显的例子表明，通过克服植物中的功能冗余，可以剖析激素信号转导和动态细胞内过程等分子机制或关键途径。由于包括更复杂的高通量筛选和快速靶标鉴定方法在内的持续进展，该领域正在开始向前发展。然而，提高自动化、成像和分析水平，并为更广泛的植物生物学界提供化学生物学资源，也是面临的挑战。
