College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, China.
Nat Prod Res. 2013;27(10):903-6. doi: 10.1080/14786419.2012.666745. Epub 2012 Mar 12.
A high-performance liquid chromatography (HPLC) technique was developed for the determination of arctigenin in plasma and various organs of rats after the oral administration of 30, 50 and 70 mgkg(-1) of arctigenin to the Sprague-Dawley rats. Results showed that the validated HPLC method was simple, fast, reproducible and suitable to the determination of arctigenin in rat plasma and organ tissue and one-compartmental model with zero-order absorption process can well describe the changes of arctigenin concentration in the plasma. The concentration of compound was highest in the spleen, less in the liver and the least in the lung.
建立了一种高效液相色谱（HPLC）法，用于测定灌胃给予大鼠 30、50 和 70mg/kg 牛蒡子苷后大鼠血浆和各组织器官中的牛蒡子苷。结果表明，该方法简便、快速、重现性好，适用于大鼠血浆和组织器官中牛蒡子苷的测定，且一室模型零级吸收过程能很好地描述牛蒡子苷在血浆中的浓度变化。化合物的浓度在脾脏中最高，在肝脏中较少，在肺中最少。
