Özalp G R, Zik B, Bastan A, Peker S, Özdemir-Salci E S, Bastan I, Darbaz I, Salar S, Karakas K
Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Uludag University, Görükle, Bursa, Turkey.
Biotech Histochem. 2012 Jul;87(5):325-30. doi: 10.3109/10520295.2012.655311. Epub 2012 Mar 12.
We investigated eight adult dogs that were brought to veterinary clinics with a history of transmissible venereal tumors (TVT). Our goal was to demonstrate the occurrence of apoptosis and the cessation of cell proliferation at every phase of scheduled chemotherapy for naturally occurring TVT. Tissue samples were collected immediately after weekly treatments with vincristine sulfate and processed for histological purposes. Sections 5 μm thick were stained by the TUNEL reaction for apoptosis and immunostained for Ki67 as a proliferation marker. We observed that after vincristine applications, tumor cell proliferation ceased and apoptosis increased. Ki67 HSCORE values were significantly lowered after the first and second treatments with the chemotherapeutic agent compared to controls, whereas TUNEL HSCORE values were significantly higher after two applications of vincristine compared to controls. Our results suggest that scheduled vincristine sulfate applications stabilize the induction of tumor regression by inducing apoptosis and preventing cell proliferation.
我们对八只因患有传染性性病肿瘤（TVT）而被送至兽医诊所的成年犬进行了研究。我们的目标是证明在针对自然发生的TVT进行的预定化疗的每个阶段，细胞凋亡的发生以及细胞增殖的停止情况。在用硫酸长春新碱每周治疗后立即采集组织样本，并进行组织学处理。将5μm厚的切片用TUNEL反应染色以检测细胞凋亡，并使用Ki67作为增殖标志物进行免疫染色。我们观察到，应用长春新碱后，肿瘤细胞增殖停止且细胞凋亡增加。与对照组相比，在使用化疗药物进行第一次和第二次治疗后，Ki67 HSCORE值显著降低，而在应用两次长春新碱后，TUNEL HSCORE值与对照组相比显著升高。我们的结果表明，预定的硫酸长春新碱应用通过诱导细胞凋亡和防止细胞增殖来稳定肿瘤消退的诱导过程。
