Department of Psychobiology, Institute of Neurosciences, Center for Biomedical Research-CIBM, University of Granada, Spain.
Behav Brain Res. 2012 May 1;230(2):418-22. doi: 10.1016/j.bbr.2012.02.038. Epub 2012 Mar 1.
Previous results indicated that damage and pharmacological inactivation of the basolateral amygdala (BLA) interfere with the attenuation of taste neophobia. A similar disruption of safe taste memories formation induced by the inhibition of protein synthesis in the perirhinal cortex (PRh) has been reported. Thus, we have assessed the effect of bilateral BLA neurotoxic lesions on PRh activity after novel and familiar taste exposure. Wistar male rats with NMDA lesions of the BLA and SHAM-operated received two consecutive exposures to a 3% cider vinegar solution. Fos-like immunoreactivity (FLI) was examined as a marker of neuronal activity in PRh. As expected the BLA lesioned group showed no evidence of neophobia attenuation. A similar number of PRh Fos-positive cells were found in SHAM and BLA groups exposed to the novel taste solution. However, the BLA-lesioned group exhibited a lower number of Fos stained cells than the SHAM-lesioned group after being exposed to the familiar taste solution. This supports the notion of BLA and PRh as components of a neural circuit involved in safe taste recognition memory and suggests a role of PRh in various forms of recognition memory.
先前的研究结果表明，破坏和药理学失活外侧杏仁核（BLA）会干扰味觉厌恶的减轻。有报道称，在旁嗅皮层（PRh）中抑制蛋白质合成也会类似地破坏安全味觉记忆的形成。因此，我们评估了双侧 BLA 神经毒性损伤对新的和熟悉的味觉暴露后 PRh 活性的影响。接受 NMDA 双侧 BLA 损伤的 Wistar 雄性大鼠和假手术（SHAM）操作的大鼠接受了两次连续的 3%苹果醋溶液暴露。作为神经元活性的标志物，检测了 Fos 样免疫反应性（FLI）。正如预期的那样，BLA 损伤组没有表现出厌恶减轻的迹象。在暴露于新的味觉溶液时，SHAM 和 BLA 组中发现了相同数量的 PRh Fos 阳性细胞。然而，与 SHAM 损伤组相比，暴露于熟悉的味觉溶液后，BLA 损伤组的 Fos 染色细胞数量较少。这支持了 BLA 和 PRh 作为参与安全味觉识别记忆的神经回路的组成部分的观点，并表明了 PRh 在各种形式的识别记忆中的作用。
