Gómez-Chacón Beatriz, Gámiz Fernando, Foster Thomas C, Gallo Milagros
Department of Psychobiology, Center for Biomedical Research (CIBM), Institute of Neurosciences, University of Granada, Avenida del Conocimiento, s/n, 18071 Granada, Spain.
Department of Neuroscience, McKnight Brain Institute, University of Florida, P.O. Box 100244, Gainesville, FL 32610-0244, USA.
Neural Plast. 2016;2016:2726745. doi: 10.1155/2016/2726745. Epub 2015 Dec 29.
Interactions between GluR2 and N-ethylmaleimide-sensitive factor (NSF) mediate AMPA receptors trafficking. This might be linked with molecular mechanisms related with memory formation. Previous research has shown basolateral amygdala (BLA) dependent activity changes in the perirhinal cortex (PRh) during the formation of taste memory. In the present experiments we investigate both the behavioral performance and the expression profile of NSF and GluR2 genes in several brain areas, including PRh, BLA, and hippocampus. Twenty-one naïve male Wistar rats were exposed to a saccharin solution (0.4%) during the first (novel), the second (Familiar I), and the sixth presentation (Familiar II). Total RNA was extracted and gene expression was measured by quantitative PCR (qPCR) using TaqMan gene expression assays. In addition the expression of the synaptic plasticity related immediate early genes, Homer 1 and Narp, was also assessed. We have found increased expression of NSF gene in BLA and PRh in Group Familiar I in comparison with Familiar II. No changes in the expression of GluR2, Homer 1, and Narp genes were found. The results suggest the relevance of a potential network in the temporal lobe for taste recognition memory and open new possibilities for understanding the molecular mechanisms mediating the impact of sensory experience on brain circuit function.
谷氨酸受体2（GluR2）与N - 乙基马来酰亚胺敏感因子（NSF）之间的相互作用介导了α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）受体的运输。这可能与记忆形成相关的分子机制有关。先前的研究表明，在味觉记忆形成过程中，基底外侧杏仁核（BLA）依赖的活动会改变嗅周皮质（PRh）的活动。在本实验中，我们研究了NSF和GluR2基因在包括PRh、BLA和海马体在内的几个脑区中的行为表现和表达谱。21只未接触过实验的雄性Wistar大鼠在第一次（新奇）、第二次（熟悉I）和第六次呈现（熟悉II）时接触糖精溶液（0.4%）。提取总RNA，并使用TaqMan基因表达分析通过定量聚合酶链反应（qPCR）测量基因表达。此外，还评估了与突触可塑性相关的即早基因Homer 1和神经元活性调节蛋白（Narp）的表达。我们发现，与熟悉II组相比，熟悉I组中BLA和PRh中NSF基因的表达增加。未发现GluR2、Homer 1和Narp基因的表达有变化。结果表明颞叶中一个潜在网络与味觉识别记忆相关，并为理解介导感觉经验对脑回路功能影响的分子机制开辟了新的可能性。
