Li J A, Lemanski L F
Department of Anatomy and Cell Biology, State University of New York, Syracuse 13210.
Anat Rec. 1990 Sep;228(1):46-52. doi: 10.1002/ar.1092280108.
Primary cultures of cardiac myocytes from normal and genetically cardiomyopathic (CM) newborn hamsters (strain UM X7.1) were analyzed by indirect immunofluorescent microscopy after 3, 5, 7, and 9 days in culture. The cultures were fixed in cold acetone and immunostained by an indirect method using FITC-labelled anti-alpha-actinin to label the myofibrillar Z bands. Most normal and CM myocytes appeared round in shape after 3 days in culture. Normal cardiac myocytes began to exhibit cytoplasmic projections after 5 days in culture and their myofibrils usually showed parallel arrangements with respect to each other. The cardiac cells from CM hearts showed an obvious myofibril disarray. Moreover, projections formed later than normal. As the size of the cells increased, more and more projections formed in normal hamster myocytes during development. By contrast, most of the cardiomyopathic myocytes showed few projections even as late as 9 days in culture. Hence, the number of projections per cell was much less in cardiomyopathic myocytes than in normal, especially after 7 and 9 days in culture. These results suggest that cardiomyopathic cells have abnormal shapes in culture and, in particular, fail to form projections as in normal cells. Whether this unusual behavior is related to an abnormality of the membranes or cytoskeletal system in cardiomyopathic heart cells or to some other factor requires further study.
对来自正常和遗传性心肌病（CM）新生仓鼠（UM X7.1品系）的心肌细胞原代培养物在培养3、5、7和9天后进行间接免疫荧光显微镜分析。培养物用冷丙酮固定，并用异硫氰酸荧光素（FITC）标记的抗α-辅肌动蛋白通过间接方法进行免疫染色，以标记肌原纤维的Z带。培养3天后，大多数正常和CM心肌细胞呈圆形。培养5天后，正常心肌细胞开始出现细胞质突起，其肌原纤维通常相互平行排列。CM心脏的心肌细胞显示出明显的肌原纤维紊乱。此外，突起形成的时间比正常细胞晚。随着细胞大小的增加，正常仓鼠心肌细胞在发育过程中形成的突起越来越多。相比之下，大多数心肌病心肌细胞即使在培养9天时也几乎没有突起。因此，心肌病心肌细胞中每个细胞的突起数量比正常细胞少得多，尤其是在培养7天和9天后。这些结果表明，心肌病细胞在培养中形状异常，特别是不能像正常细胞那样形成突起。这种异常行为是否与心肌病心脏细胞中的膜或细胞骨架系统异常或其他因素有关，需要进一步研究。
