Messina D A, Lemanski L F
Department of Anatomy and Cell Biology, Suny, Syracuse 13210.
J Mol Cell Cardiol. 1994 Jul;26(7):937-41. doi: 10.1006/jmcc.1994.1111.
In the present study, we compare fetal, newborn and adult normal and cardiomyopathic hearts for the presence of spectrin. Spectrin is a cytoskeletal-membrane protein found associated with the membranes and cortical regions of a variety of cell types. Its function in non-erythroid tissue is not fully understood. We determined previously that spectrin is associated with the membranes, myofibrils, intercalated discs and possibly the T-tubules of developing normal Syrian hamsters. Thus, it is a good candidate for comparison with cardiomyopathic (CM) Syrian hamster hearts since this model system exhibits myofibril and membrane abnormalities. Normal and CM hamsters were analysed at three stages of fetal development, as well as newborn, and adult stages using immunohistochemical, electron microscopic, and electrophoretic techniques. The data presented here indicate that no obvious difference exists in the distribution of spectrin between normal and CM hearts. Thus, the isoform of spectrin recognized by our antibody with the present protocols is not affected by the cardiomyopathic condition and can be ruled out as a contributor to the myofibril disarray and membrane-associated defects seen in these animals.
在本研究中，我们比较了胎儿、新生儿和成人的正常心脏及心肌病心脏中血影蛋白的存在情况。血影蛋白是一种细胞骨架膜蛋白，存在于多种细胞类型的细胞膜和皮质区域。其在非红细胞组织中的功能尚未完全明确。我们先前已确定，血影蛋白与发育中的正常叙利亚仓鼠的细胞膜、肌原纤维、闰盘以及可能的横小管相关。因此，由于该模型系统存在肌原纤维和膜异常，它是与心肌病（CM）叙利亚仓鼠心脏进行比较的良好候选对象。使用免疫组织化学、电子显微镜和电泳技术，在胎儿发育的三个阶段以及新生儿和成年阶段对正常和CM仓鼠进行了分析。此处呈现的数据表明，正常心脏和CM心脏中血影蛋白的分布没有明显差异。因此，按照本实验方案，我们的抗体识别的血影蛋白同工型不受心肌病状态的影响，并且可以排除其是导致这些动物出现肌原纤维紊乱和膜相关缺陷的因素。
