Madhulika N, Rangdhol R Vishwanath, Sitra G, Ballaiah John, Jaikumar R Arun, Brooklyin S
Department of Oral Medicine, Indira Gandhi Institute of Dental Sciences, Puducherry, India.
Department of Prosthodontics, Rajah Mutiah Dental College, Chidambaram, Tamil Nadu, India.
J Pharm Bioallied Sci. 2015 Aug;7(Suppl 2):S451-6. doi: 10.4103/0975-7406.163499.
This study aims to quantify the extent of DNA damage in lymphocytes of patients with oral lichen planus (OLP) and oral lichenoid reactions (OLRs) using comet assay.
Lymphocytes from peripheral blood were subjected to alkaline comet assay. Comet length (CL), head diameter (HD), percentage of DNA in head, tail length (TL), percentage of DNA in tail, tail intensity, tail mean and tail moment were compared between study group (OLP and OLR) and control group using Student's t-test. Pearson's correlation coefficient was used to examine the linear association between the variables.
Significantly higher levels of DNA damage was present in study group as reflected by CL, HD and TL, tail intensity and tail moment with P = 0.0001; percentage of DNA in head and tail with P = 0.02 and tail mean with P = 0.012.
This study brings out the fact that DNA damage measured by comet assay was greater in the study group when compared to the control group. As a reflection of uniqueness, this study crowns the scenario with respect to early detection and prevention of potentially malignant disorders and the process of malignant transformation.
本研究旨在通过彗星试验量化口腔扁平苔藓（OLP）和口腔苔藓样反应（OLR）患者淋巴细胞中的DNA损伤程度。
对外周血淋巴细胞进行碱性彗星试验。使用学生t检验比较研究组（OLP和OLR）与对照组之间的彗星长度（CL）、头部直径（HD）、头部DNA百分比、尾部长度（TL）、尾部DNA百分比、尾部强度、尾部均值和尾部矩。使用Pearson相关系数检验变量之间的线性关联。
研究组中DNA损伤水平显著更高，表现为CL、HD和TL、尾部强度和尾部矩，P = 0.0001；头部和尾部DNA百分比，P = 0.02；尾部均值，P = 0.012。
本研究表明，与对照组相比，研究组中通过彗星试验测得的DNA损伤更大。作为独特性的体现，本研究在潜在恶性疾病的早期检测和预防以及恶性转化过程方面堪称典范。
