Biochemistry Division, Department of Chemistry, University of Pune, Pune 411007, India.
Appl Microbiol Biotechnol. 2013 Jan;97(2):881-9. doi: 10.1007/s00253-012-3983-6. Epub 2012 Mar 11.
Alishewanella sp. strain KMK6 was able to degrade mixture of textile dyes (0.5-2.0 g l(-1)) within 8 h. An initial 28 % reduction in COD was observed immediately after decolorization at static anoxic conditions which on further incubation at shaking conditions reduced by 90 %. Partially purified azoreductase was able to utilize different azo dyes as substrates. The HPLC profile of dye degradation showed formation of metabolic products. Further FTIR analysis showed significant changes in the peaks corresponding to functional groups present in dye mixture and its degradation products. The genotoxicity assessment showed that the dye degradation products were non-toxic compared to dye mixture.
希瓦氏菌（Alishewanella）sp. 菌株 KMK6 能够在 8 小时内降解混合纺织染料（0.5-2.0 g/L）。在静态缺氧条件下脱色后，COD 立即降低了 28%，而在进一步在振荡条件下孵育时，COD 降低了 90%。部分纯化的偶氮还原酶能够利用不同的偶氮染料作为底物。HPLC 染料降解图谱显示形成了代谢产物。进一步的 FTIR 分析表明，染料混合物及其降解产物中存在的功能基团的峰发生了显著变化。遗传毒性评估表明，与染料混合物相比，染料降解产物的毒性较低。
