Kolekar Yogesh M, Kodam Kisan M
Biochemistry Division, Department of Chemistry, University of Pune, Pune, India.
Appl Microbiol Biotechnol. 2012 Jul;95(2):521-9. doi: 10.1007/s00253-011-3698-0. Epub 2011 Nov 17.
Alishewanella sp. strain KMK6 was isolated from textile dye-contaminated soil. The strain was able to decolorize and degrade different azo dyes and displayed high dye degradation ability and tolerance. The bacterium could completely degrade 2.5 g l(-1) dye, Reactive Blue 59 within 6 h. The induction in the level of cytochrome P-450 and activities of azoreductase and NADH-dichlorophenolindophenol reductase were observed in the cells after dye decolorization indicating the role of these enzymes. The intermediates of Reactive Blue 59 degradation were identified by high-performance liquid chromatography, gas chromatography and mass spectroscopy, and Fourier transform infrared spectroscopy. The ecotoxicity has been evaluated for dye and its metabolites by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (a yellow tetrazole) and comet assay, and it revealed that the dye degradation products were nontoxic.
希瓦氏菌属菌株KMK6是从受纺织染料污染的土壤中分离出来的。该菌株能够使不同的偶氮染料脱色并降解，表现出较高的染料降解能力和耐受性。该细菌能够在6小时内完全降解2.5 g l⁻¹的活性蓝59染料。在染料脱色后，观察到细胞色素P-450水平以及偶氮还原酶和NADH-二氯酚靛酚还原酶活性的诱导，表明这些酶发挥了作用。通过高效液相色谱、气相色谱和质谱以及傅里叶变换红外光谱法鉴定了活性蓝59降解的中间产物。通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（一种黄色四氮唑）和彗星试验评估了染料及其代谢产物的生态毒性，结果表明染料降解产物无毒。
