Tianjin Institute of Urology, Department of Urology, Tianjin 300211, China.
Cancer Biol Ther. 2012 May;13(7):527-33. doi: 10.4161/cbt.19602. Epub 2012 May 1.
We investigated the role of vasculogenic mimicry (VM) in the progression of prostate cancer (PCa). Ninety-six patients who had undergone prostatectomy for treatment of PCa and for whom a complete record of clinical and follow-up data were available were reviewed. VM and matrix metalloproteinase-2 (MMP-2) were detected by immunohistochemical staining in frozen tissue sections. Relationship between VM and clinicopathological variables was analyzed statistically. Multivariate analyses were performed to assess the prognostic significance of VM. Results showed that out of the 96 PCa cases studied here, VM was detectable in 24 (25%) and was positively correlated with preoperative prostate-specific antigen (PSA) level, Gleason score, pathological stage, lymph node metastasis, seminal vesicle invasion, distant metastasis and PSA doubling time (PSADT). Univariate analysis showed that VM, PSA level, Gleason score, distant metastasis and PSADT were correlated with overall survival (OS), while VM, Gleason score, distant metastasis, local recurrence and PSADT were correlated with disease-free survival (DFS). Multivariable analysis indicated that the presence of VM, higher Gleason score and distant metastasis were the adverse predictors of OS and DFS. A higher widespread staining for MMP-2 was correlated with the VM-positive subgroup. In conclusion, VM mainly exists in the high risk PCa patients and is an independent marker of poor prognosis.
我们研究了血管生成拟态（VM）在前列腺癌（PCa）进展中的作用。回顾了 96 名接受前列腺切除术治疗 PCa 的患者的资料，这些患者的临床和随访数据完整。通过免疫组织化学染色在冷冻组织切片中检测 VM 和基质金属蛋白酶-2（MMP-2）。统计分析 VM 与临床病理变量之间的关系。进行多变量分析以评估 VM 的预后意义。结果表明，在研究的 96 例 PCa 病例中，24 例（25%）可检测到 VM，与术前前列腺特异性抗原（PSA）水平、Gleason 评分、病理分期、淋巴结转移、精囊侵犯、远处转移和 PSA 倍增时间（PSADT）呈正相关。单因素分析表明，VM、PSA 水平、Gleason 评分、远处转移和 PSADT 与总生存期（OS）相关，而 VM、Gleason 评分、远处转移、局部复发和 PSADT 与无病生存期（DFS）相关。多变量分析表明，VM 的存在、较高的 Gleason 评分和远处转移是 OS 和 DFS 的不良预测因子。MMP-2 的广泛高染色与 VM 阳性亚组相关。总之，VM 主要存在于高危 PCa 患者中，是预后不良的独立标志物。
