从嗜热真菌里氏木霉中克隆、大肠杆菌中过表达并鉴定一种耐热木聚糖乙酰酯酶。
Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii.
机构信息
Molecular Glycobiotechnology Group, Biochemistry Department, School of Natural Sciences, National University of Ireland Galway, Galway, Ireland.
出版信息
Appl Environ Microbiol. 2012 May;78(10):3759-62. doi: 10.1128/AEM.05659-11. Epub 2012 Mar 9.
Abstract
The gene encoding an acetylxylan esterase (AXE1) from the thermophilic ascomycete Talaromyces emersonii was cloned, expressed in Escherichia coli, and characterized. This form of AXE1, rTeAXE1, exhibits increased thermostability and activity at a higher temperature than other known fungal acetyl esterases, thus having huge potential application in biomass bioconversion to high value chemicals or biofuels.
摘要
从嗜热子囊菌塔宾曲霉中克隆、在大肠杆菌中表达并鉴定了编码乙酰木聚糖酯酶（AXE1）的基因。与其他已知真菌乙酰酯酶相比，这种形式的 AXE1（rTeAXE1）在更高温度下表现出更高的热稳定性和活性，因此在生物质生物转化为高价值化学品或生物燃料方面具有巨大的潜在应用。
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